BMRB Entry 28000

Title:
Structural basis for client recognition and activity of Hsp40 chaperones
Deposition date:
2019-08-16
Original release date:
2019-11-07
Authors:
jiang, yajun; rossi, paolo; kalodimos, charalampos
Citation:

Citation: Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos. "Structural basis for client recognition and activity of Hsp40 chaperones"  Science 365, 1313-1319 (2019).
PubMed: 31604242

Assembly members:

Assembly members:
Ydj1-SBD, polymer, 240 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet16b

Data sets:
Data typeCount
15N chemical shifts217
1H chemical shifts217

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ydj1_SBD1

Entities:

Entity 1, Ydj1_SBD 240 residues - Formula weight is not available

1   ARGGLYLYSASPILELYSHISGLUILESER
2   ALASERLEUGLUGLULEUTYRLYSGLYARG
3   THRALALYSLEUALALEUASNLYSGLNILE
4   LEUCYSLYSGLUCYSGLUGLYARGGLYGLY
5   LYSLYSGLYALAVALLYSLYSCYSTHRSER
6   CYSASNGLYGLNGLYILELYSPHEVALTHR
7   ARGGLNMETGLYPROMETILEGLNARGPHE
8   GLNTHRGLUCYSASPVALCYSHISGLYTHR
9   GLYASPILEILEASPPROLYSASPARGCYS
10   LYSSERCYSASNGLYLYSLYSVALGLUASN
11   GLUARGLYSILELEUGLUVALHISVALGLU
12   PROGLYMETLYSASPGLYGLNARGILEVAL
13   PHELYSGLYGLUALAASPGLNALAPROASP
14   VALILEPROGLYASPVALVALPHEILEVAL
15   SERGLUARGPROHISLYSSERPHELYSARG
16   ASPGLYASPASPLEUVALTYRGLUALAGLU
17   ILEASPLEULEUTHRALAILEALAGLYGLY
18   GLUPHEALALEUGLUHISVALSERGLYASP
19   TRPLEULYSVALGLYILEVALPROGLYGLU
20   VALILEALAPROGLYMETARGLYSVALILE
21   GLUGLYLYSGLYMETPROILEPROLYSTYR
22   GLYGLYTYRGLYASNLEUILEILELYSPHE
23   THRILELYSASPPROGLUASNHISPHETHR
24   SERGLUGLUASNLEULYSLYSLEUGLUGLU

Samples:

sample_1: Ydj1-SBD, [U-13C; U-15N; U-2H], 400 uM; TRIS 20 mM; potassium chloride 75 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCOCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks