BMRB Entry 27999

Title:
Structural basis for client recognition and activity of Hsp40 chaperones
Deposition date:
2019-08-16
Original release date:
2019-11-07
Authors:
Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos
Citation:

Citation: Jiang, Yajun; Rossi, Paolo; Kalodimos, Charalampos. "Structural basis for client recognition and activity of Hsp40 chaperones"  Science 365, 1313-1319 (2019).
PubMed: 31604242

Assembly members:

Assembly members:
Sis1_SBD, polymer, 158 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet16b

Data sets:
Data typeCount
15N chemical shifts138
1H chemical shifts138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sis1_SBD1

Entities:

Entity 1, Sis1_SBD 158 residues - Formula weight is not available

1   GLUGLUGLUTHRVALGLNVALASNLEUPRO
2   VALSERLEUGLUASPLEUPHEVALGLYLYS
3   LYSLYSSERPHELYSILEGLYARGLYSGLY
4   PROHISGLYALASERGLULYSTHRGLNILE
5   ASPILEGLNLEULYSPROGLYTRPLYSALA
6   GLYTHRLYSILETHRTYRLYSASNGLNGLY
7   ASPTYRASNPROGLNTHRGLYARGARGLYS
8   THRLEUGLNPHEVALILEGLNGLULYSSER
9   HISPROASNPHELYSARGASPGLYASPASP
10   LEUILETYRTHRLEUPROLEUSERPHELYS
11   GLUSERLEULEUGLYPHESERLYSTHRILE
12   GLNTHRILEASPGLYARGTHRLEUPROLEU
13   SERARGVALGLNPROVALGLNPROSERGLN
14   THRSERTHRTYRPROGLYGLNGLYMETPRO
15   THRPROLYSASNPROSERGLNARGGLYASN
16   LEUILEVALLYSTYRLYSVALASP

Samples:

sample_1: Sis1_SBD, [U-100% 13C; U-100% 15N], 700 uM; potassium chloride 75 mM; potassium phosphate 20 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks