BMRB Entry 27946

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for RA-bound Cellular Retinoic Acid Binding Protein 2
Deposition date:
2019-06-17
Original release date:
2019-11-08
Authors:
Lixa, Carolina; Iqbal, Anwar; Almeida, Fabio; Pinheiro, Anderson
Citation:

Citation: Lixa, Carolina; Clarkson, Michael; Iqbal, Anwar; Moon, Thomas; Almeida, Fabio; Peti, Wolfgang; Pinheiro, Anderson. "Retinoic Acid Binding Leads to CRABP2 Rigidification and Dimerization"  Biochemistry 58, 4183-4194 (2019).
PubMed: 31566355

Assembly members:

Assembly members:
CRABP2, polymer, 140 residues, Formula weight is not available
RETINOIC ACID, non-polymer, 300.435 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: RP1B

Data sets:
Data typeCount
13C chemical shifts343
15N chemical shifts126
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CRABP2 monomer1
2RETINOIC ACID2

Entities:

Entity 1, CRABP2 monomer 140 residues - Formula weight is not available

1   GLYHISMETPROASNPHESERGLYASNTRP
2   LYSILEILEARGSERGLUASNPHEGLUGLU
3   LEULEULYSVALLEUGLYVALASNVALMET
4   LEUARGLYSILEALAVALALAALAALASER
5   LYSPROALAVALGLUILELYSGLNGLUGLY
6   ASPTHRPHETYRILELYSTHRSERTHRTHR
7   VALARGTHRTHRGLUILEASNPHELYSVAL
8   GLYGLUGLUPHEGLUGLUGLNTHRVALASP
9   GLYARGPROCYSLYSSERLEUVALLYSTRP
10   GLUSERGLUASNLYSMETVALCYSGLUGLN
11   LYSLEULEULYSGLYGLUGLYPROLYSTHR
12   SERTRPTHRARGGLULEUTHRASNASPGLY
13   GLULEUILELEUTHRMETTHRALAASPASP
14   VALVALCYSTHRARGVALTYRVALARGGLU

Entity 2, RETINOIC ACID - C20 H28 O2 - 300.435 Da.

1   REA

Samples:

sample_1: RA-bound CRABP2, [U-13C; U-15N], 1 mM; NaP 20 mM; NaCl 150 mM; DTT 5 mM; retinoic acid 1 mM

sample_conditions_1: ionic strength: 0.170 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks