BMRB Entry 27836

Title:
Backbone 1H, 15N, and 13C Chemical Shifts of Myosin VI Medial Tail Domain
Deposition date:
2019-03-14
Original release date:
2019-06-26
Authors:
Barnes, C. Ashley; Shen, Yang; Ying, Jinfa; Takagi, Yasuharu; Torchia, Dennis; Sellers, James; Bax, Ad
Citation:

Citation: Barnes, C. Ashley; Shen, Yang; Ying, Jinfa; Takagi, Yasuharu; Torchia, Dennis; Sellers, James; Bax, Ad. "Remarkable Rigidity of the Single alpha-Helical Domain of Myosin-VI As Revealed by NMR Spectroscopy."  J. Am. Chem. Soc. 141, 9004-9017 (2019).
PubMed: 31117653

Assembly members:

Assembly members:
MT_Domain, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: pig   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Sus scrofa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts118
15N chemical shifts67
1H chemical shifts67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MT Domain1

Entities:

Entity 1, MT Domain 68 residues - Formula weight is not available

1   LYSGLNGLNGLUGLUGLUALAGLUARGLEU
2   ARGARGILEGLNGLUGLUMETGLULYSGLU
3   ARGLYSARGARGGLUGLUASPGLUGLNARG
4   ARGARGLYSGLUGLUGLUGLUARGARGMET
5   LYSLEUGLUMETGLUALALYSARGLYSGLN
6   GLUGLUGLUGLUARGLYSLYSARGGLUASP
7   ASPGLULYSARGILEGLNALAGLU

Samples:

sample_1: sodium phosphate 20 mM; EDTA 2 mM; MT Domain, [U-13C; U-15N; U-2H], 1 mM

sample_2: sodium phosphate 20 mM; EDTA 2 mM; MT Domain, [U-100% 2H; U-100% 15N], 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.3; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
4D HN-HN NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, peak picking, processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks