BMRB Entry 27793

Title:
NMR resonance assignments of the T102R mutant of the EVH1 domain of neurofibromin's recruitment factor Spred1
Deposition date:
2019-02-20
Original release date:
2019-03-15
Authors:
Fuehrer, Sebastian; Tollinger, Martin; Dunzendorfer-Matt, Theresia
Citation:

Citation: Fuehrer, Sebastian; Tollinger, Martin; Dunzendorfer-Matt, Theresia. "Binding mode of a Legius syndrome patient derived mutant form of the EVH1 domain of Neurofibromin s recruitment factor Spred1"  J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
T102R_Spred1-EVH1, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21d

Data sets:
Data typeCount
13C chemical shifts343
15N chemical shifts120
1H chemical shifts125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1T102R Spred1-EVH11

Entities:

Entity 1, T102R Spred1-EVH1 120 residues - Formula weight is not available

The two first residues, Met-Gly, are derived from the plasmid

1   METGLYSERTYRALAARGVALARGALAVAL
2   VALMETTHRARGASPASPSERSERGLYGLY
3   TRPLEUPROLEUGLYGLYSERGLYLEUSER
4   SERVALTHRVALPHELYSVALPROHISGLN
5   GLUGLUASNGLYCYSALAASPPHEPHEILE
6   ARGGLYGLUARGLEUARGASPLYSMETVAL
7   VALLEUGLUCYSMETLEULYSLYSASPLEU
8   ILETYRASNLYSVALTHRPROTHRPHEHIS
9   HISTRPLYSILEASPASPLYSLYSPHEGLY
10   LEUARGPHEGLNSERPROALAASPALAARG
11   ALAPHEASPARGGLYILEARGARGALAILE
12   GLUASPILESERGLNGLYCYSPROGLUSER

Samples:

sample_1: T102R Spred1-EVH1, [U-99% 13C; U-99% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Agilent DD2 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks