BMRB Entry 27700

Title:
Human linker histone NGH1x in low ionic strength conditions
Deposition date:
2018-11-21
Original release date:
2019-01-02
Authors:
De Wit, Herna; Vallet, Alicia; Brutscher, Bernhard; Koorsen, Gerrit
Citation:

Citation: De Wit, Herna; Vallet, Alicia; Brutscher, Bernhard; Koorsen, Gerrit; De Wit, Herna; Vallet, Alicia; Brutscher, Bernhard; Koorsen, Gerrit. "NMR assignments of human linker histone H1x N-terminal domain and globular domain in the presence and absence of perchlorate"  Biomol. NMR Assign. 13, 249-254 (2019).
PubMed: 30868366

Assembly members:

Assembly members:
NGH1x_no_salt, polymer, 119 residues, 13101.1949 Da.

Natural source:

Natural source:   Common Name: NGH1x   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b+

Data typeCount
13C chemical shifts307
15N chemical shifts107
1H chemical shifts107

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NGH1x no salt1

Entities:

Entity 1, NGH1x no salt 119 residues - 13101.1949 Da.

1   METSERVALGLULEUGLUGLUALALEUPRO
2   VALTHRTHRALAGLUGLYMETALALYSLYS
3   VALTHRLYSALAGLYGLYSERALAALALEU
4   SERPROSERLYSLYSARGLYSASNSERLYS
5   LYSLYSASNGLNPROGLYLYSTYRSERGLN
6   LEUVALVALGLUTHRILEARGARGLEUGLY
7   GLUARGASNGLYSERSERLEUALALYSILE
8   TYRTHRGLUALALYSLYSVALPROTRPPHE
9   ASPGLNGLNASNGLYARGTHRTYRLEULYS
10   TYRSERILELYSALALEUVALGLNASNASP
11   THRLEULEUGLNVALLYSGLYTHRGLYALA
12   ASNGLYSERPHELYSLEUASNARGLYS

Samples:

NGH1x_low_ionic_strength: NGH1x_no_salt, [U-99% 13C; U-99% 15N], 0.5 mM

CondSet1: ionic strength: 20 mM; pH: 7.000; temperature: 278.000 K

CondSet4: pH: 7.0; temperature: 278 K

CondSet13: pH: 7.0; temperature: 278 K

CondSet14: pH: 7.0; temperature: 278 K

CondSet15: pH: 7.0; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCNGH1x_low_ionic_strengthisotropicCondSet1
3D HNCONGH1x_low_ionic_strengthisotropicCondSet4
3D HNCACBNGH1x_low_ionic_strengthisotropicCondSet13
HNCOCACB (H[N[co[{CA|ca[C]}]]])NGH1x_low_ionic_strengthisotropicCondSet14
HNCACO (H[N[ca[CO]]])NGH1x_low_ionic_strengthisotropicCondSet15

Software:

CcpNmr_Analysis v2.4, CCPN - Spectral analysis

NMR spectrometers:

  • Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks