BMRB Entry 27623

Title:
Klebsiella pneumoniae sigma4 of sigma70 fused to the beta-flap-tip helix
Deposition date:
2018-09-24
Original release date:
2019-08-15
Authors:
Lou, Yuan-Chao; Chen, Chinpan
Citation:

Citation: Lou, Yuan-Chao; Chou, Chun-Chi; Yeh, Hsin-Hong; Chien, Chia-Yu; Sadotra, Sushant; Hsu, Chun-Hua; Chen, Chinpan. "Structural basis for -35 element recognition by sigma4 chimera proteins and their interactions with PmrA response regulator"  Proteins 88, 69-81 (2020).
PubMed: 31293000

Assembly members:

Assembly members:
sigma4-70c, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Klebsiella pneumoniae   Taxonomy ID: 573   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Klebsiella pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet-GB1

Data sets:
Data typeCount
13C chemical shifts377
15N chemical shifts92
1H chemical shifts476

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sigma4-70c monomer1

Entities:

Entity 1, sigma4-70c monomer 108 residues - Formula weight is not available

1   GLYLEUPROLEUASPSERALATHRTHRGLU
2   SERLEUARGALAALATHRHISASPVALLEU
3   ALAGLYLEUTHRALAARGGLUALALYSVAL
4   LEUARGMETARGPHEGLYILEASPMETASN
5   THRASPHISTHRLEUGLUGLUVALGLYLYS
6   GLNPHEASPVALTHRARGGLUARGILEARG
7   GLNILEGLUALALYSALALEUARGLYSLEU
8   ARGHISPROSERARGSERGLUVALLEUARG
9   SERPHELEUGLYSERGLYTRPLYSGLYGLU
10   THRGLNLEUTHRPROGLUGLULYSLEULEU
11   ARGALAILEPHEGLYGLULYSALA

Samples:

sample_1: sigma4-70c, [U-99% 13C; U-99% 15N], 1.2 mM; Na2HPO4 20 mM; NaCl 30 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks