BMRB Entry 27570

Title:
Chemical shift assignment of wild-type E.coli diacylglycerol kinase (DGK) by solid-state NMR
Deposition date:
2018-08-06
Original release date:
2019-02-26
Authors:
Moebius, Kristin; Kazemi, Sina; Guentert, Peter; Jakob, Andreas; Heckel, Alexander; Becker-Baldus, Johanna; Glaubitz, Clemens
Citation:

Citation: Moebius, Kristin; Kazemi, Sina; Guentert, Peter; Jakob, Andreas; Becker-Baldus, Johanna; Glaubitz, Clemens. "Global response of wild-type E.coli diacylglycerol kinase towards nucleotide and lipid substrate binding observed by 3D and 2D MAS NMR"  Sci. Rep. 9, 3995-3995 (2019).
PubMed: 30850624

Assembly members:

Assembly members:
DGK, polymer, 131 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSD005

Data sets:
Data typeCount
13C chemical shifts418
15N chemical shifts112
1H chemical shifts5

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DGK, chain 11
2DGK, chain 21
3DGK, chain 31

Entities:

Entity 1, DGK, chain 1 131 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISGLULEU
2   ALAASNASNTHRTHRGLYPHETHRARGILE
3   ILELYSALAALAGLYTYRSERTRPLYSGLY
4   LEUARGALAALATRPILEASNGLUALAALA
5   PHEARGGLNGLUGLYVALALAVALLEULEU
6   ALAVALVALILEALACYSTRPLEUASPVAL
7   ASPALAILETHRARGVALLEULEUILESER
8   SERVALMETLEUVALMETILEVALGLUILE
9   LEUASNSERALAILEGLUALAVALVALASP
10   ARGILEGLYSERGLUTYRHISGLULEUSER
11   GLYARGALALYSASPMETGLYSERALAALA
12   VALLEUILEALAILEILEVALALAVALILE
13   THRTRPCYSILELEULEUTRPSERHISPHE
14   GLY

Samples:

sample_1: DGK, [U-13C; U-15N], mM

sample_2: DGK, [U-13C; U-15N]; [U-12C; U-14N]-Ile,Leu,Val, mM

sample_conditions_1: pH: 7.2; temperature: 275 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
3D CONCAsample_1solidsample_conditions_1
3D NCACXsample_2solidsample_conditions_1
3D NCOCXsample_2solidsample_conditions_1
3D CONCAsample_2solidsample_conditions_1
2D 13C-13C TOBSYsample_1solidsample_conditions_1
2D 1H-13C HETCORsample_1solidsample_conditions_1
2D 1H-15N HETCORsample_1solidsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

ssFLYA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

CCPN, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 850 MHz