BMRB Entry 27464

Title:
C-terminal tail of Protein Phosphatase 1, alpha isoform.
Deposition date:
2018-04-23
Original release date:
2019-04-26
Authors:
Bajaj, Rakhi; Page, Rebecca; Peti, Wolfgang
Citation:

Citation: Bertran, M Teresa; Mouilleron, Stephane; Zhou, Yanxiang; Bajaj, Rakhi; Uliana, Federico; Kumar, Ganesan Senthil; van Drogen, Audrey; Lee, Rebecca; Banerjee, Jennifer; Hauri, Simon; O'Reilly, Nicola; Gstaiger, Matthias; Page, Rebecca; Peti, Wolfgang; Tapon, Nicolas. "ASPP proteins discriminate between PP1 catalytic subunits through their SH3 domain and the PP1 C-tail"  Nat. Commun. 10, 771-771 (2019).
PubMed: 30770806

Assembly members:

Assembly members:
PP1_C-_ter, polymer, 34 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-M30-MBP

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PP1_C-_ter: GAMGKNKGKYGQFSGLNPGG RPITPPRNSAKAKK

Data sets:
Data typeCount
13C chemical shifts53
15N chemical shifts27
1H chemical shifts27

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein phosphatse 11

Entities:

Entity 1, Protein phosphatse 1 34 residues - Formula weight is not available

Residue 1 -4 GAMG is cloning artifact and residues 5-34 correspond to residues 301-330 of of Protein phosphatase 1 ( alpha isoform).

1   GLYALAMETGLYLYSASNLYSGLYLYSTYR
2   GLYGLNPHESERGLYLEUASNPROGLYGLY
3   ARGPROILETHRPROPROARGASNSERALA
4   LYSALALYSLYS

Samples:

sample_1: PP1 C- ter, [U-99% 13C; U-99% 15N], 0.4 mM; Bis-Tris pH=6.5 20 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance-NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks