BMRB Entry 27317

Title:
Solid-state NMR assignment of HBV core protein
Deposition date:
2017-11-24
Original release date:
2018-03-07
Authors:
Lecoq, Lauriane; Wang, Shishan; Wiegand, Thomas; Bressanelli, Stephane; Nassal, Michael; Bockmann, Anja; Meier, Beat
Citation:

Citation: Lecoq, Lauriane; Wang, Shishan; Wiegand, Thomas; Bressanelli, Stephane; Nassal, Michael; Meier, Beat; Bockmann, Anja. "Solid-state [13C-15N] NMR resonance assignment of Hepatitis B Virus core protein"  Biomol. NMR Assignments 12, 205-214 (2018).
PubMed: 29450824

Assembly members:

Assembly members:
Cp149, polymer, 149 residues, 17777 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a2

Data sets:
Data typeCount
13C chemical shifts621
15N chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cp149, 11
2Cp149, 21
3Cp149, 31
4Cp149, 41

Entities:

Entity 1, Cp149, 1 149 residues - 17777 Da.

Residues 1-149 of the full-length protein Cp183 containing 183 residues.

1   METASPILEASPPROTYRLYSGLUPHEGLY
2   ALATHRVALGLULEULEUSERPHELEUPRO
3   SERASPPHEPHEPROSERVALARGASPLEU
4   LEUASPTHRALASERALALEUTYRARGGLU
5   ALALEUGLUSERPROGLUHISCYSSERPRO
6   HISHISTHRALALEUARGGLNALAILELEU
7   CYSTRPGLYGLULEUMETTHRLEUALATHR
8   TRPVALGLYVALASNLEUGLUASPPROALA
9   SERARGASPLEUVALVALSERTYRVALASN
10   THRASNMETGLYLEULYSPHEARGGLNLEU
11   LEUTRPPHEHISILESERCYSLEUTHRPHE
12   GLYARGGLUTHRVALILEGLUTYRLEUVAL
13   SERPHEGLYVALTRPILEARGTHRPROPRO
14   ALATYRARGPROPROASNALAPROILELEU
15   SERTHRLEUPROGLUTHRTHRVALVAL

Samples:

sample_1: Hepatitis B Virus capsid, [U-100% 13C; U-100% 15N], 50 mg/mL

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARR 20mssample_1isotropicsample_conditions_1
2D 13C-13C DARR 200mssample_1isotropicsample_conditions_1
2D NCAsample_1isotropicsample_conditions_1
2D NCOsample_1isotropicsample_conditions_1
3D NCACBsample_1isotropicsample_conditions_1
3D NCACXsample_1isotropicsample_conditions_1
3D CANCOsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
3D CCCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP P03146
AlphaFold P03146