BMRB Entry 27099

Title:
Chemical shift assignment of gp17.1 by proton-detected solid-state NMR
Deposition date:
2017-05-08
Original release date:
2017-06-29
Authors:
Zinke, Maximilian; Fricke, Pascal; Lange, Sascha; Lange, Adam
Citation:

Citation: Zinke, Maximilian; Fricke, Pascal; Samson, Camille; Hwang, Songhwan; Wall, Joseph; Lange, Sascha; Zinn-Justin, Sophie; Lange, Adam. "Bacteriophage Tail Tube Assembly Studied by Proton-Detected 4D Solid-State NMR"  Angew. Chem. Int. Ed. Engl. 56, 9497-9501 (2017).
PubMed: 28644511

Assembly members:

Assembly members:
gp17-1, polymer, 185 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus phage SPP1   Taxonomy ID: 10724   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lambdavirus SPP1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM13

Data sets:
Data typeCount
13C chemical shifts460
15N chemical shifts151
1H chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gp17.11

Entities:

Entity 1, gp17.1 185 residues - Formula weight is not available

1   METGLYMETPROGLUTHRPROILEMETGLY
2   GLNASPVALLYSTYRLEUPHEGLNSERILE
3   ASPALAALATHRGLYSERALAPROLEUPHE
4   PROALATYRGLNTHRASPGLYSERVALSER
5   GLYGLUARGGLULEUPHEASPGLUGLNTHR
6   LYSASNGLYARGILELEUGLYPROGLYSER
7   VALALAASPSERGLYGLUVALTHRTYRTYR
8   GLYLYSARGGLYASPALAGLYGLNLYSALA
9   ILEGLUASPALATYRGLNASNGLYLYSGLN
10   ILELYSPHETRPARGVALASPTHRVALLYS
11   ASNGLUASNASPLYSTYRASPALAGLNPHE
12   GLYPHEALATYRILEGLUSERARGGLUTYR
13   SERASPGLYVALGLUGLYALAVALGLUILE
14   SERILESERLEUGLNVALILEGLYGLULEU
15   LYSASNGLYGLUILEASPTHRLEUPROGLU
16   GLUILEVALASNVALSERLYSGLYGLYTYR
17   ASPPHEGLNGLNPROGLYGLNTHRTHRGLY
18   GLUALAPROGLYTHRVALPROALAPROHIS
19   HISHISHISHISHIS

Samples:

sample_1: gp17.1, [U-13C; U-15N; U-2H]; 100% back-exchange, 13 mg

sample_conditions_1: ionic strength: 0.5 M; pH: 7.4; pressure: 1 atm; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
2D (H)NHsample_1isotropicsample_conditions_1
3D (H)CANHsample_1isotropicsample_conditions_1
3D (H)CNONHsample_1isotropicsample_conditions_1
3D (H)N(CACO)NHsample_1isotropicsample_conditions_1
4D (H)COCANHsample_1isotropicsample_conditions_1
4D (H)CBCANHsample_1isotropicsample_conditions_1
4D (H)CACONHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks