BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25952

Title: 1H, 13C, and 15N chemical shift assignments of the free lectin from Ralstonia solanacearum   PubMed: 26845253

Authors: Antonik, Pawel; van Nuland, Nico; Volkov, Alexander; Crowley, Peter

Citation: Antonik, Pawel; Volkov, Alexander; Broder, Ursula; Re, Daniele; van Nuland, Nico; Crowley, Peter. "Anomer-Specific Recognition and Dynamics in a Fucose-Binding Lectin"  Biochemistry 55, 1195-1203 (2016).

Assembly members:
RSL, polymer, 90 residues, Formula weight is not available

Natural source:   Common Name: b-proteobacteria   Taxonomy ID: 305   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Ralstonia solanacearum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RSL: SSVQTAATSWGTVPSIRVYT ANNGKITERCWDGKGWYTGA FNEPGDNVSVTSWLVGSAIH IRVYASTGTTTTEWCWDGNG WTKGAYTATN

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts96
1H chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RSL1

Entities:

Entity 1, RSL 90 residues - Formula weight is not available

1   SERSERVALGLNTHRALAALATHRSERTRP
2   GLYTHRVALPROSERILEARGVALTYRTHR
3   ALAASNASNGLYLYSILETHRGLUARGCYS
4   TRPASPGLYLYSGLYTRPTYRTHRGLYALA
5   PHEASNGLUPROGLYASPASNVALSERVAL
6   THRSERTRPLEUVALGLYSERALAILEHIS
7   ILEARGVALTYRALASERTHRGLYTHRTHR
8   THRTHRGLUTRPCYSTRPASPGLYASNGLY
9   TRPTHRLYSGLYALATYRTHRALATHRASN

Samples:

sample_1: RSL, [U-100% 13C; U-100% 15N], 2 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 68 mM; pH: 6.1; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN, Vranken et al. - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian Uniform NMR System 600 MHz

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