BMRB Entry 25688

Title:
Novel Structural Components Contribute to the High Thermal Stability of Acyl Carrier Protein from Enterococcus faecalis
Deposition date:
2015-07-02
Original release date:
2015-12-07
Authors:
Park, Young-Guen; Jung, Min-Cheol; Song, Hee Sang; Jeong, Ki-Woong; Kim, Yangmee
Citation:

Citation: Park, Young-Guen; Jung, Min-Cheol; Song, Hee Sang; Jeong, Ki-Woong; Bang, Eunjung; Hwang, Geum-Sook; Kim, Yangmee. "Novel Structural Components Contribute to the High Thermal Stability of Acyl Carrier Protein from Enterococcus faecalis"  J. Biol. Chem. 291, 1692-1702 (2016).
PubMed: 26631734

Assembly members:

Assembly members:
entity, polymer, 79 residues, 8707.609 Da.

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: not available   Superkingdom: Bacteria   Kingdom: Eubacteria   Genus/species: Enterococcus faecalis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-11a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts229
15N chemical shifts78
1H chemical shifts511

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 79 residues - 8707.609 Da.

1   METTHRARGGLUGLUVALLEUGLNLYSVAL
2   ALALYSILEILESERASNHISPHEASPILE
3   GLUALAASPGLNVALTHRASPGLNLEUASN
4   ILELYSASPASPLEUASNALAASPSERILE
5   SERVALMETGLUPHEVALLEUGLULEUGLU
6   ASPGLUPHEGLYTHRGLUILESERASPGLU
7   ASPALAGLULYSILEGLUTHRVALGLYALA
8   ALAVALASPTYRILEVALSERASNSER

Samples:

efACP-holo: ENTITY, [U-100% 13C; U-100% 15N], mM; MES 25 mM; Cacl2 5 mM; DTT 5 mM; D2O, [U-99% 2H], 10%; H2O 90%

sample_conditions_1: pH: 6.1; pressure: 1 atm; temperature: 25 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCefACP-holoisotropicsample_conditions_1
2D 1H-1H NOESYefACP-holoisotropicsample_conditions_1
3D CBCA(CO)NHefACP-holoisotropicsample_conditions_1
3D HNCOefACP-holoisotropicsample_conditions_1
3D HNCACBefACP-holoisotropicsample_conditions_1
3D HCCH-TOCSYefACP-holoisotropicsample_conditions_1
3D 1H-15N NOESYefACP-holoisotropicsample_conditions_1
3D 1H-13C NOESYefACP-holoisotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks