BMRB Entry 25610

Title:
NMR solution structure of the pheromone Ep-1 from Euplotes petzi
Deposition date:
2015-05-14
Original release date:
2016-04-25
Authors:
Pedrini, Bill; Vallesi, Adriana; Alimenti, Claudio; Luporini, Pierangelo
Citation:

Citation: Pedrini, Bill; Suter-Stahel, Thea; Vallesi, Adriana; Alimenti, Claudio; Luporini, Pierangelo. "Molecular Structures and Coding Genes of the Water-Borne Protein Pheromones of Euplotes petzi, an Early Diverging Polar Species of Euplotes"  J. Eukaryot. Microbiol. 64, 164-172 (2017).
PubMed: 27455370

Assembly members:

Assembly members:
Ep-1, polymer, 32 residues, 3180.605 Da.

Natural source:

Natural source:   Common Name: ciliates   Taxonomy ID: 1156992   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Euplotes petzi

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Euplotes petzi

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ep-1: SCGSECAPEPDCWGCCLVQC APSICAGWCGGS

Data sets:
Data typeCount
1H chemical shifts163

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 32 residues - 3180.605 Da.

1   SERCYSGLYSERGLUCYSALAPROGLUPRO
2   ASPCYSTRPGLYCYSCYSLEUVALGLNCYS
3   ALAPROSERILECYSALAGLYTRPCYSGLY
4   GLYSER

Samples:

sample_1: Ep-1 0.25 ± 0.05 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 0.06 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al., Keller and Wuthrich - chemical shift assignment, refinement

ATNOS, Guentert, P, Herrmann, T, Wuethrich, K - peak picking

CANDID, Guentert, P, Herrmann, T, Wuethrich, K - peak assignment, upper distance constraint determination

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz