BMRB Entry 25516

Title:
Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic alpha-Conotoxin Vc1.1
Deposition date:
2015-03-04
Original release date:
2016-04-12
Authors:
Yu, Rilei; Seymour, Victoria; Berecki, G za; Jia, Xinying; Akcan, Muharrem; Adams, David; Kaas, Quentin; Craik, David
Citation:

Citation: Yu, Rilei; Seymour, Victoria; Berecki, G za; Jia, Xinying; Akcan, Muharrem; Adams, David; Kaas, Quentin; Craik, David. "Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic alpha-Conotoxin Vc1.1"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
entity, polymer, 22 residues, 2263.418 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GHCSDPRFNYDHPEICGGAA GG

Data sets:
Data typeCount
13C chemical shifts13
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 22 residues - 2263.418 Da.

1   GLYHISCYSSERASPPROARGPHEASNTYR
2   ASPHISPROGLUILECYSGLYGLYALAALA
3   GLYGLY

Samples:

sample_1: cVc 1.1 mutant 1.7 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 4.5; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

TOPSPIN, Bruker Biospin - collection, processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz