BMRB Entry 25274

Title:
Solution structure of cytosolic part of Trop2
Deposition date:
2014-10-08
Original release date:
2015-05-04
Authors:
Ilc, Gregor; Plavec, Janez; Vidmar, Tilen; Pavsic, Miha; Lenarcic, Brigita
Citation:

Citation: Ilc, Gregor; Lenarcic, Brigita; Plavec, Janez; Vidmar, Tilen; Pavsic, Miha. "A Structural View of the Trop2-Mediated Signaling Mechanism Revealed by NMR and Molecular Dynamics"  .

Assembly members:

Assembly members:
entity, polymer, 26 residues, 3096.678 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: TNRRKSGKYKKVEIKELGEL RKEPSL

Data sets:
Data typeCount
13C chemical shifts74
15N chemical shifts21
1H chemical shifts186

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 26 residues - 3096.678 Da.

1   THRASNARGARGLYSSERGLYLYSTYRLYS
2   LYSVALGLUILELYSGLULEUGLYGLULEU
3   ARGLYSGLUPROSERLEU

Samples:

sample_1: TropIC 5 mM; Trifluoroethanol; H2O

sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian VNMRS 800 MHz

Related Database Links:

BMRB 19342
PDB
EMBL CAA31781 CAA54799 CAA54801 CAG47056
GB AAA52505 AAH09409 AAP35919 AAP88905 AAX29730
REF NP_002344 XP_002810812 XP_003265191 XP_003824268 XP_003892008
SP P09758
AlphaFold P09758

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks