BMRB Entry 25038

Title:
Structural Investigation of hnRNP L
Deposition date:
2014-06-24
Original release date:
2015-05-04
Authors:
Blatter, Markus; Allain, Frederic
Citation:

Citation: Blatter, Markus; Allain, Frederic. "Structural Investigation of hnRNP L"  To be Published ., .-..

Assembly members:

Assembly members:
entity, polymer, 105 residues, 11386.674 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTYB11

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts103
1H chemical shifts677

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 105 residues - 11386.674 Da.

1   GLYGLUASNTYRASPASPPROHISLYSTHR
2   PROALASERPROVALVALHISILEARGGLY
3   LEUILEASPGLYVALVALGLUALAASPLEU
4   VALGLUALALEUGLNGLUPHEGLYPROILE
5   SERTYRVALVALVALMETPROLYSLYSARG
6   GLNALALEUVALGLUPHEGLUASPVALLEU
7   GLYALACYSASNALAVALASNTYRALAALA
8   ASPASNGLNILETYRILEALAGLYHISPRO
9   ALAPHEVALASNTYRSERTHRSERGLNLYS
10   ILESERARGPROGLYASPSERASPASPSER
11   ARGSERVALASNSER

Samples:

sample_1: entity, [U-100% 15N], 1-2 mM; sodium chloride 60 mM; sodium phosphate 40 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_2: entity, [U-100% 13C; U-100% 15N], 1-2 mM; sodium chloride 60 mM; sodium phosphate 40 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_3: entity, [U-100% 15N], 1-2 mM; sodium chloride 60 mM; sodium phosphate 40 mM; DTT 1 mM; D2O 100%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 310.15 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY, Goddard - chemical shift assignment

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAB18649 BAC39565 BAE02304 BAE26011 BAF84804
EMBL CAA34261
GB AAH27206 AAH69184 AAH99683 AAI69048 EAW56826
PRF 1604358A
REF NP_001005335 NP_001128232 NP_001178959 NP_001252929 NP_001267082
SP P14866 Q8R081
TPG DAA19831
AlphaFold P14866 Q8R081

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks