BMRB Entry 25032

Title:
Solution structure of a bacterial immunoglobulin-like domain form a surface protein of Leptospira
Deposition date:
2014-06-19
Original release date:
2014-07-14
Authors:
Mei, Song
Citation:

Citation: Mei, Song. "Solution structure of a bacterial immunoglobulin-like domain form a surface protein of Leptospira"  .

Assembly members:

Assembly members:
entity, polymer, 102 residues, 10426.439 Da.

Natural source:

Natural source:   Common Name: spirochetes   Taxonomy ID: 44276   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Leptospira interrogans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PQE30

Data sets:
Data typeCount
1H chemical shifts487
13C chemical shifts193
15N chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1surface protein of Leptospira1

Entities:

Entity 1, surface protein of Leptospira 102 residues - 10426.439 Da.

1   GLUASNILEASPILETHRVALSERALAALA
2   THRLEUSERSERILESERILESERPROILE
3   ASNTHRASNILEASNTHRTHRVALSERLYS
4   GLNPHEPHEALAVALGLYTHRTYRSERASP
5   GLYTHRLYSALAASPLEUTHRSERSERVAL
6   THRTRPSERSERSERASNGLNSERGLNALA
7   LYSVALSERASNALASERGLUTHRLYSGLY
8   LEUVALTHRGLYILEALASERGLYASNPRO
9   THRILEILEALATHRTYRGLYSERVALSER
10   GLYASNTHRILELEUTHRVALASNLYSTHR
11   ASPTHR

Samples:

sample_1: H2O 90%; D2O 10%

sample_conditions_1: temperature: 293 K; pH: 6.5; pressure: 1 atm; ionic strength: 100 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 19942
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks