BMRB Entry 20112

Name: CHARACTERIZATION OF THE LIGAND-FREE CONSTITUTIVE ACTIVITY OF THE THROMBOXANE A2 RECEPTOR BY THE INTEGRATED APPROACHES OF NMR SPECTROSCOPY AND A RECOMBINANT RECEPTOR
Published: 2010-01-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20112

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CHARACTERIZATION OF THE LIGAND-FREE CONSTITUTIVE ACTIVITY OF THE THROMBOXANE A2 RECEPTOR BY THE INTEGRATED APPROACHES OF NMR SPECTROSCOPY AND A RECOMBINANT RECEPTOR

Deposition date:

2009-12-16

Original release date:

2010-01-22

Authors:

Chillar, Annirudha; Wu, Jiaxin; Cervantes, Vanessa; Ruan, Ke-He

Citation:

Citation: Chillar, Annirudha; Wu, Jiaxin; Cervantes, Vanessa; Ruan, Ke-He. "Structural and functional analysis of the C-terminus of Galphaq in complex with the human thromboxane A2 receptor provides evidence of constitutive activity" Biochemistry 49, 6365-6374 (2010).
PubMed: 20590159

Assembly members:

Assembly members:
Gaq-Ct_peptide, polymer, 15 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Gaq-Ct_peptide: KDTILQLNLKEYNLV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
conformer_family_coord_set
- ensemble_coordinates

Data type	Count
1H chemical shifts	112
conformer coordinate sets	1

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Caq-ct	1

Entities:

Entity 1, Caq-ct 15 residues - Formula weight is not available

1

LYS

ASP

THR

ILE

LEU

GLN

LEU

ASN

LEU

LYS

2

GLU

TYR

ASN

LEU

VAL

Samples:

sample_1: (Gaq-Ct peptide) 2.2 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

FELIX v2000, Accelrys Software Inc. - chemical shift assignment, chemical shift calculation, data analysis, peak picking, processing, refinement, structure solution

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	20109 20118
EMBL	CAG08422
GB	ACF47673 ACF47675 EGW10015 EGW13773 EHB02791
REF	XP_004048208 XP_004059801 XP_005431300 XP_005642455 XP_008508489