BMRB Entry 20047

Title:
INTERACTION OF THE DENGUE VIRUS FUSION PEPTIDE WITH MEMBRANES ASSESSED BY NMR: THE ESSENTIAL ROLE OF THE ENVELOPE PROTEIN TRP101 FOR MEMBRANE FUSION
Deposition date:
2008-09-25
Original release date:
2009-07-23
Authors:
Melo, Manuel; Sousa, Francisco; Carneiro, Fabiana; Castanho, Miguel; Valente, Ana Paula; Almeida, Fabio; da Poian, Andrea; Mohana-Borges, Ronaldo
Citation:

Citation: Melo, Manuel; Sousa, Francisco; Carneiro, Fabiana; Castanho, Miguel; Valente, Ana Paula; Almeida, Fabio; da Poian, Andrea; Mohana-Borges, Ronaldo. "Interaction of the dengue virus fusion peptide with membranes assessed by NMR: The essential role of the envelope protein TRP101 for membrane fusion."  J. Mol. Biol. 392, 736-746 (2009).
PubMed: 19619560

Assembly members:

Assembly members:
fusionpeptide, polymer, 15 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Dengue virus   Taxonomy ID: 12637   Superkingdom: Viruses   Kingdom: not available   Genus/species: Flavivirus Dengue virus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fusionpeptide: DRGWGNGCGLFGKGG

Data sets:
Data typeCount
1H chemical shifts71

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1fusiopeptide1

Entities:

Entity 1, fusiopeptide 15 residues - Formula weight is not available

This is the fusion peptide of dengue glycoprotein E

1   ASPARGGLYTRPGLYASNGLYCYSGLYLEU
2   PHEGLYLYSGLYGLY

Samples:

DPC_micelles: fusionpeptide 2 mM; DPC, [U-99% 2H], 100 mM; DTT, [U-99% 2H], 10 mM; sodium phosphate 100 mM; sodium azide 0.01%; D2O, [U-99% 2H], 10%

sample_conditions: pH: 5.5; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYDPC_micellesisotropicsample_conditions
2D 1H-1H NOESYDPC_micellesisotropicsample_conditions

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, chemical shift calculation, peak picking

CNSSOLVE v1.0, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA00176 BAA00254 BAA00255 BAA00393 BAA00394
EMBL CAA27184 CAA27332 CAA33284 CAA33474 CAA33475
GB AAA17498 AAA17499 AAA17500 AAA17501 AAA17502
PIR C32401 GNWVDF GNWVS5
PRF 1303269A 1412347A 1611496A 1613132A 1613132B
REF NP_041724 NP_041726 NP_051124 NP_056776 NP_059433
SP P03314 P05769 P06935 P07564 P09732
AlphaFold P06935 P09732 P07564 P05769 P03314