BMRB Entry 20031

Title:
13C, 1H chemical shifts and structure of Apelin 17 (Human form, minor conformation at 5C)
Deposition date:
2008-07-03
Original release date:
2009-02-04
Authors:
Langelaan, David; Bebbington, E.; Reddy, Tyler; Rainey, Jan
Citation:

Citation: Langelaan, David; Bebbington, E.; Reddy, Tyler; Rainey, Jan. "Structural insight into G-protein coupled receptor binding by apelin"  Biochemistry 48, 537-548 (2009).
PubMed: 19123778

Assembly members:

Assembly members:
Apelin_17, polymer, 17 residues, 2145.619 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Apelin_17: KFRRQRPRLSHKGPMPF

Data sets:
Data typeCount
1H chemical shifts145
13C chemical shifts60
coupling constants12

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Apelin 171

Entities:

Entity 1, Apelin 17 17 residues - 2145.619 Da.

1   LYSPHEARGARGGLNARGPROARGLEUSER
2   HISLYSGLYPROMETPROPHE

Samples:

Apelin_17_sample: Apelin 17 4 ± 0.4 mM; DSS 1 mM; sodium azide 1 mM; Sodium Acetate, [U-99% 2H], 20 mM

5C: pH: 5; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCApelin_17_sampleisotropic5C
2D 1H-1H TOCSYApelin_17_sampleisotropic5C
2D 1H-1H NOESYApelin_17_sampleisotropic5C
2D DQF-COSYApelin_17_sampleisotropic5C

Software:

VNMR, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 16275 20029 20030 20082
DBJ BAA84974 BAA84975 BAA84976 BAA84977 BAC31156
EMBL CAC83678
GB AAF25814 AAF25815 AAH20015 AAH21104 AAH80843
REF NP_001177797 NP_038940 NP_059109 NP_113800 NP_776928
SP Q9R0R3 Q9R0R4 Q9TUI9 Q9ULZ1
TPG DAA13389
AlphaFold Q9TUI9 Q9ULZ1 Q9R0R4 Q9R0R3