BMRB Entry 19878

Title:
Structure of Platypus 'Intermediate' Defensin-like Peptide (Int-DLP)
Deposition date:
2014-03-27
Original release date:
2014-09-15
Authors:
Torres, Allan; Bansal, Paramjit; Koh, Jennifer; Pages, Guilhem; Wu, Ming; Kuchel, Philip
Citation:

Citation: Torres, Allan; Bansal, Paramjit; Koh, Jennifer; Pages, Guilhem; Wu, Ming; Kuchel, Philip. "Structure and antimicrobial activity of platypus 'intermediate' defensin-like peptide"  FEBS Lett. 588, 1821-1826 (2014).
PubMed: 24694388

Assembly members:

Assembly members:
Int-DLP, polymer, 44 residues, 5000.910 Da.

Natural source:

Natural source:   Common Name: platypus   Taxonomy ID: 9258   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ornithorhynchus anatinus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Int-DLP: RRRRRRPPCEDVNGQCQPRG NPCLRLRGACPRGSRCCMPT VAAH

Data sets:
Data typeCount
1H chemical shifts216

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Int-DLP1

Entities:

Entity 1, Int-DLP 44 residues - 5000.910 Da.

1   ARGARGARGARGARGARGPROPROCYSGLU
2   ASPVALASNGLYGLNCYSGLNPROARGGLY
3   ASNPROCYSLEUARGLEUARGGLYALACYS
4   PROARGGLYSERARGCYSCYSMETPROTHR
5   VALALAALAHIS

Samples:

sample_1: Int-DLP1 – 2 mM

sample_conditions_1: temperature: 298 K; pH: 3.5; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, data analysis, structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UniProtKB P0C8B1
PDB
SP P0C8B1
AlphaFold P0C8B1