BMRB Entry 19834

Title:
New Cyt-like delta-endotoxins from Dickeya dadantii - CytC protein
Deposition date:
2014-03-05
Original release date:
2015-02-16
Authors:
Loth, Karine; Costechareyre, Denis; Effantin, Geraldine; Rahbe, Yvan; Contemine, Guy; Landon, Celine; Da Silva, Pedro
Citation:

Citation: Loth, Karine; Costechareyre, Denis; Effantin, Geraldine; Rahbe, Yvan; Contdemine, Guy; Landon, Celine; Da Silva, Pedro. "New Cyt-like delta-endotoxins from Dickeya dadantii : structure and aphicidal activity"  JBC ., .-..

Assembly members:

Assembly members:
entity, polymer, 203 residues, 22412.430 Da.

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 204038   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Dickeya dadantii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Data sets:
Data typeCount
13C chemical shifts727
15N chemical shifts215
1H chemical shifts1161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 203 residues - 22412.430 Da.

1   METSERILEGLUILETYRPROASPASPGLY
2   ASNTHRLEUPROTYRGLNVALPHELEUASN
3   LEUGLUASNGLUHISTYRTYRALAGLNALA
4   ILEGLNLEUALAGLNLEUPHEALAHISGLU
5   VALASPASPASNGLYGLNLEUASPLEUALA
6   LYSALALEULYSLYSALAGLNALAGLNPRO
7   ASPLEUALAILEILEALATHRASNASNMET
8   THRLEULYSLYSSERPHESERTHRLEUSER
9   ALALEUTHRTHRTHRLEUSERGLUGLNLEU
10   LYSILEGLUGLYVALLEUGLYILESERGLN
11   ASPTHRTYRILEGLNASNILELEUSERHIS
12   ALAPHECYSASPLEUGLUTHRGLNLYSASP
13   LYSPROTRPPHEHISILEASNALAGLNPRO
14   ASPALAGLYHISSERVALTHRSERTYRSER
15   TYRSERLEUPHEILEVALSERGLNGLYGLU
16   GLUTHRGLYALAMETMETALAALAGLYPRO
17   LEUILEILETHRVALTHRPROASNTHRALA
18   ILESERASPILEPHEASNTHRLYSASPTRP
19   ARGLEUTHRLEUGLNLYSGLUGLUILETHR
20   ILEGLYVALLYSGLYPHEGLNVALVALTHR
21   PROLEUGLY

Samples:

sample_1: CytC, [U-13C; U-15N], 0.3 mM; TRIS 50 mM; sodium chloride 100 mM; EDTA 1 mM; DTT 5 mM; EDTA 90%; DTT 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

CcpNMR v2.1.5, CCPN - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ARIA v2.3, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB ADM96668 ERO59479
REF WP_013316150 WP_022631959 WP_038900160 WP_038910128 WP_038921535

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks