BMRB Entry 19823
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19823
MolProbity Validation Chart
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Zimmermann, Marcel; Hegemann, Julian; Xie, Xiulan; Marahiel, Mohamed. "Characterization of caulonodin lasso peptides revealed unprecedented N-terminal residues and a precursor motif essential for peptide maturation" Chem. Sci. 5, 4032-4043 (2014).
Assembly members:
entity, polymer, 18 residues, 2002.157 Da.
Natural source: Common Name: a-proteobacteria Taxonomy ID: 366602 Superkingdom: Bacteria Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET41A
Entity Sequences (FASTA):
entity: SIGDSGLRESMSSQTYWP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 110 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lasso Peptide Caulonodin V | 1 |
Entities:
Entity 1, Lasso Peptide Caulonodin V 18 residues - 2002.157 Da.
| 1 | SER | ILE | GLY | ASP | SER | GLY | LEU | ARG | GLU | SER | ||||
| 2 | MET | SER | SER | GLN | THR | TYR | TRP | PRO |
Samples:
sample_1: cnd5 10 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB |