BMRB Entry 19667

Title:
Solution structure of the carboxyterminal domain of NusG from Mycobacterium tuberculosis
Deposition date:
2013-12-09
Original release date:
2014-01-27
Authors:
Strauss, Martin; Schweimer, Kristian; Roesch, Paul
Citation:

Citation: Strauss, Martin; Schweimer, Kristian; Roesch, Paul. "Solution structure of the carboxyterminal domain of NusG from mycobacterium tuberculosis"  .

Assembly members:

Assembly members:
entity, polymer, 62 residues, 6711.688 Da.

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET101/D-TOPO

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts263
15N chemical shifts60
1H chemical shifts446

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1carboxyterminal domain of NusG1

Entities:

Entity 1, carboxyterminal domain of NusG 62 residues - 6711.688 Da.

1   GLYARGPROVALVALGLUVALASPTYRGLU
2   VALGLYGLUSERVALTHRVALMETASPGLY
3   PROPHEALATHRLEUPROALATHRILESER
4   GLUVALASNALAGLUGLNGLNLYSLEULYS
5   VALLEUVALSERILEPHEGLYARGGLUTHR
6   PROVALGLULEUTHRPHEGLYGLNVALSER
7   LYSILE

Samples:

sample_1: entity, [U-95% 13C; U-90% 15N], 1.1 mM; HEPES 25 mM; sodium chloride 150 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMRView, Johnson, One Moon Scientific - NMR data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAH24951 BAL64520 BAQ04534 GAA44419
EMBL CAL70674 CCC25720 CCC42978 CCC63246 CCE36182
GB AAK44893 ABQ72374 ABR04995 ACT23685 AEB02783
REF NP_215153 NP_854316 WP_003403288 WP_023349453 WP_023370810
SP P65590 P9WIU8 P9WIU9
AlphaFold P9WIU9 P65590 P9WIU8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks