BMRB Entry 19637

Title:
NMR solution Structure of the E.coli Outer Membrane Protein W
Deposition date:
2013-11-26
Original release date:
2014-08-04
Authors:
Horst, R.; Stanczak, P.; Wuthrich, K
Citation:

Citation: Horst, Reto; Stanczak, Pawel; Wuthrich, Kurt. "NMR polypeptide backbone conformation of the E. coli outer membrane protein W."  Structure 22, 1204-1209 (2014).
PubMed: 25017731

Assembly members:

Assembly members:
Outer_Membrane_Protein_W, polymer, 192 residues, 21002.574 Da.

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
1H chemical shifts177
13C chemical shifts470
15N chemical shifts177

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Outer Membrane Protein X from E. coli1

Entities:

Entity 1, Outer Membrane Protein X from E. coli 192 residues - 21002.574 Da.

1   METHISGLUALAGLYGLUPHEPHEMETARG
2   ALAGLYSERALATHRVALARGPROTHRGLU
3   GLYALAGLYGLYTHRLEUGLYSERLEUGLY
4   GLYPHESERVALTHRASNASNTHRGLNLEU
5   GLYLEUTHRPHETHRTYRMETALATHRASP
6   ASNILEGLYVALGLULEULEUALAALATHR
7   PROPHEARGHISLYSILEGLYTHRARGALA
8   THRGLYASPILEALATHRVALHISHISLEU
9   PROPROTHRLEUMETALAGLNTRPTYRPHE
10   GLYASPALASERSERLYSPHEARGPROTYR
11   VALGLYALAGLYILEASNTYRTHRTHRPHE
12   PHEASPASNGLYPHEASNASPHISGLYLYS
13   GLUALAGLYLEUSERASPLEUSERLEULYS
14   ASPSERTRPGLYALAALAGLYGLNVALGLY
15   VALASPTYRLEUILEASNARGASPTRPLEU
16   VALASNMETSERVALTRPTYRMETASPILE
17   ASPTHRTHRALAASNTYRLYSLEUGLYGLY
18   ALAGLNGLNHISASPSERVALARGLEUASP
19   PROTRPVALPHEMETPHESERALAGLYTYR
20   ARGPHE

Samples:

sample_1: Outer Membrane Protein W, [U-13C; U-15N; U-2H], 1.2 mM; H2O 90%; D2O 10%; Sodium Chloride 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.8; pressure: 1 bar; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
NOESY/HNCA/HNCACBsample_1isotropicsample_conditions_1
HNCO/HNCOCA/HNCACOsample_1isotropicsample_conditions_1

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA14788 BAG66417 BAG76829 BAJ43052 BAL38320
EMBL CAA31922 CAP75801 CAQ31757 CAQ98135 CAR12762
GB AAB60065 AAB60073 AAB60081 AAB60089 AAB60097
REF NP_415772 NP_707165 WP_000737220 WP_000737221 WP_000737222
SP P0A915 P0A916
AlphaFold P0A916 P0A915

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks