BMRB Entry 19619

Title:
Structure and NMR assignments of lantibiotic NAI-107 in DPC micelles
Deposition date:
2013-11-18
Original release date:
2014-03-03
Authors:
Munch, Daniela; Muller, Anna; Schneider, Tanja; Kohl, Bastian; Wenzel, Michaela; Bandow, Julia; Maffioli, Sonia; Sosio, Margherita; Donadio, Stefano; Wimmer, Reinhard; Sahl, Hans-Georg
Citation:

Citation: Munch, Daniela; Muller, Anna; Schneider, Tanja; Kohl, Bastian; Wenzel, Michaela; Bandow, Julia Elisabeth; Maffioli, Sonia; Sosio, Margherita; Donadio, Stefano; Wimmer, Reinhard; Sahl, Hans-Georg. "The Lantibiotic NAI-107 Binds to Bactoprenol-bound Cell Wall Precursors and Impairs Membrane Functions."  J. Biol. Chem. 289, 12063-12076 (2014).
PubMed: 24627484

Assembly members:

Assembly members:
entity_1, polymer, 24 residues, 2003.355 Da.

Natural source:

Natural source:   Common Name: Microbispora sp. 107891   Taxonomy ID: 415003   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Microbispora Microbispora sp. 107891

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Microbispora   Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: VXXXXLCXPGCTXXGGGXNC XFCX

Data sets:
Data typeCount
13C chemical shifts40
1H chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lantibiotic NAI-1071

Entities:

Entity 1, lantibiotic NAI-107 24 residues - 2003.355 Da.

1   VALDBUDAL5CWDHALEUCYSDBBPROGLY
2   CYSTHRDALHYPGLYGLYGLYDALASNCYS
3   DALPHECYSTEE

Samples:

sample_1: entity_1 3.5 mM; dodecylphosphocholine-d38, [U-2H], 150 mM; potassium phosphate 10 mM; TSP-d4, 2,2,3,3-D, 0.02 mM

sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 310.1 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

YASARA, Yasara Biosciences - refinement

NMR spectrometers:

  • Bruker AVIII 600 MHz
  • Bruker Avance 900 MHz
  • Bruker DRX 600 MHz