BMRB Entry 19381

Title:
Engineering G4: Towards effective incorporation of locked nucleic acid into G-quadruplexes
Deposition date:
2013-07-22
Original release date:
2015-02-02
Authors:
Li, Zhe; Lech, Christopher; Adrian, Michael; Heddi, Brahim; Phan, Anh Tuan
Citation:

Citation: Li, Zhe; Lech, Christopher; Adrian, Michael; Heddi, Brahim; Phan, Anh Tuan. "Engineering G4: Towards effective incorporation of locked nucleic acid into G-quadruplexes"  .

Assembly members:

Assembly members:
DNA_(5'-D(*TP*TP*GP*LGP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*A)-3'), polymer, 24 residues, 7607.944 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA_(5'-D(*TP*TP*GP*LGP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*A)-3'): TTGXGTTAGGGTTAGGGTTA GGGA

Data sets:
Data typeCount
1H chemical shifts186

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA/RNA (5'-D(*TP*TP*G)-R(P*(LG))-D(P*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*A)-3')1

Entities:

Entity 1, DNA/RNA (5'-D(*TP*TP*G)-R(P*(LG))-D(P*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*A)-3') 24 residues - 7607.944 Da.

1   DTDTDGLCGDGDTDTDADGDG
2   DGDTDTDADGDGDGDTDTDA
3   DGDGDGDA

Samples:

sample_1: DNA (5'-D(*TP*TP*GP*LGP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*A)-3') 1.5 mM; DSS 20 uM; potassium phosphate 20 mM; H2O 90%; D2O 10%

sample_2: DNA (5'-D(*TP*TP*GP*LGP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*A)-3') 1.5 mM; DSS 20 uM; potassium phosphate 20 mM; D2O 100%

sample_conditions_1: ionic strength: 30 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz