BMRB Entry 19250

Title:
STRUCTURE OF LASSO PEPTIDE ASTEXIN3
Deposition date:
2013-05-18
Original release date:
2013-07-29
Authors:
Maksimov, Mikhail; Link, A. James
Citation:

Citation: Maksimov, Mikhail; Link, A. James. "Discovery and characterization of an isopeptidase that linearizes lasso peptides."  J. Am. Chem. Soc. 135, 12038-12047 (2013).
PubMed: 23862624

Assembly members:

Assembly members:
ASTEXIN3, polymer, 24 residues, 2542.775 Da.

Natural source:

Natural source:   Common Name: ASTICCACAULIS EXCENTRICUS   Taxonomy ID: 78587   Superkingdom: BACTERIA   Kingdom: not available   Genus/species: ASTICCACAULIS EXCENTRICUS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI   Vector: PASK-75

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ASTEXIN3: GPTPMVGLDSVSGQYWDQHA PLAD

Data sets:
Data typeCount
1H chemical shifts155

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASTEXIN31

Entities:

Entity 1, ASTEXIN3 24 residues - 2542.775 Da.

1   GLYPROTHRPROMETVALGLYLEUASPSER
2   VALSERGLYGLNTYRTRPASPGLNHISALA
3   PROLEUALAASP

Samples:

sample: ASTEXIN3 16.25 mg/mL

sample_conditions_1: temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsampleisotropicsample_conditions_1
2D NOESYsampleisotropicsample_conditions_1
2D COSYsampleisotropicsample_conditions_1

Software:

CYANA v6.0, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN v2.1, Ponder - processing

MESTRENOVA v7.0.3, MestreLab Research, S.S.L., Santiago de Compostella, Spain - processing

TINKER v6.0, Ponder - refinement

NMR spectrometers:

  • BRUKER AVANCE-III 500 MHz

Related Database Links:

BMRB 25783
PDB
GB ADU14098