BMRB Entry 18951

Title:
NMR solution structure of a MYB-like DNA binding domain of KNL-2 from C. Elegans
Deposition date:
2013-01-15
Original release date:
2014-03-31
Authors:
Osborne, Michael; Borden, Katherine
Citation:

Citation: De Rop, Valerie; Dorn, Jonas; Osborne, Michael; Boisvert, Jacques; Ryan, Joel; Padeganeh, Abbas; Moevus, Corentin; Borden, Katherine; Maddox, Amy; Maddox, Paul. "Centromere epigenome stability is mediated by structural recognition by the Centromere Licensing Complex"  Science ., .-..

Assembly members:

Assembly members:
KNL2, polymer, 134 residues, 15216.123 Da.

Natural source:

Natural source:   Common Name: Caenorhabditis elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P1

Data sets:
Data typeCount
13C chemical shifts170
15N chemical shifts47
1H chemical shifts351

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MYB-like DNA binding domain of KNL-2 from C. Elegans1

Entities:

Entity 1, MYB-like DNA binding domain of KNL-2 from C. Elegans 134 residues - 15216.123 Da.

Residues 1-5 (GPLGS) are a cloning artefact

1   GLYPROLEUGLYSERVALALALYSLYSILE
2   THRTRPARGLYSGLNASPLEUASPARGLEU
3   LYSARGVALILEALALEULYSLYSPROSER
4   ALASERASPALAASPTRPTHRGLUVALLEU
5   ARGLEULEUALALYSGLUGLYVALVALGLU
6   PROGLUVALVALARGGLNILEALAILETHR
7   ARGLEULYSTRPVALGLUPROGLYPROLEU
8   GLYSERVALALALYSLYSILETHRTRPARG
9   LYSGLNASPLEUASPARGLEULYSARGVAL
10   ILEALALEULYSLYSPROSERALASERASP
11   ALAASPTRPTHRGLUVALLEUARGLEULEU
12   ALALYSGLUGLYVALVALGLUPROGLUVAL
13   VALARGGLNILEALAILETHRARGLEULYS
14   TRPVALGLUPRO

Samples:

sample_1: KNL2, [U-99% 13C; U-99% 15N], 0.2 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 2 mM; sodium azide 0.02%; Magnesium Sulphate 5 mM

sample_2: KNL2, [U-99% 13C; U-99% 15N], 0.2 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 2 mM; sodium azide 0.02%; Magnesium Sulphate 5 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1

Software:

CYANA v3,0, Guntert, Mumenthaler and Wuthrich - automated structure calculation

NMRView, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks