BMRB Entry 18836

Title:
Backbone assignments of 8M urea denatured S-YibK-C variant from Haemophilus influenzae (HI0766)
Deposition date:
2012-11-12
Original release date:
2019-08-30
Authors:
Hsieh, Shu-Ju Micky; Chien, Chih-Ta Henry; Hsu, Shang-Te Danny
Citation:

Citation: Hsieh, Shu-Ju Micky; Mallam, Anna; Jackson, Sophie; Hsu, Shang-Te Danny. "Backbone 1H, 13C and 15N assignments of YibK and avariant containing a unique cysteine residue at C-terminus in 8 M urea-denatured states"  Biomol. NMR Assignments 8, 439-442 (2014).
PubMed: 23853076

Assembly members:

Assembly members:
S-YibK-C, polymer, 167 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 727   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Haemophilus influenzae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet 17b

Data sets:
Data typeCount
13C chemical shifts387
15N chemical shifts145
1H chemical shifts283

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S-YibK-C1

Entities:

Entity 1, S-YibK-C 167 residues - Formula weight is not available

1   METSERGLYSERMETLEUASPILEVALLEU
2   TYRGLUPROGLUILEPROGLNASNTHRGLY
3   ASNILEILEARGLEUALAALAASNTHRGLY
4   PHEARGLEUHISLEUILEGLUPROLEUGLY
5   PHETHRTRPASPASPLYSARGLEUARGARG
6   SERGLYLEUASPTYRHISGLUPHEALAGLU
7   ILELYSARGHISLYSTHRPHEGLUALAPHE
8   LEUGLUSERGLULYSPROLYSARGLEUPHE
9   ALALEUTHRTHRLYSGLYALAPROALAHIS
10   SERGLNVALLYSPHELYSLEUGLYASPTYR
11   LEUMETPHEGLYPROGLUTHRARGGLYILE
12   PROMETSERILELEUASNGLUMETPROMET
13   GLUGLNLYSILEARGILEPROMETTHRALA
14   ASNSERARGSERMETASNLEUSERASNSER
15   VALALAVALTHRVALTYRGLUALATRPARG
16   GLNLEUGLYTYRLYSGLYALAVALASNLEU
17   PROGLUVALLYSGLYSERCYS

Samples:

sample_1: S-YibK-C, [U-13C; U-15N], 350 uM; D2O 10%; TRIS 50 mM; potassium chloride 200 mM; glycerol 10%; urea 8 M; H2O 90%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks