BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18684

Title: Identification of the Structural Traits Mediating the Antimicrobial Activity of a Chimeric Peptide of HBD2 and HBD3   PubMed: 22995312

Authors: Spudy, Bjorn; Soennichsen, Frank; Waetzig, Georg; Groetzinger, Joachim; Jung, Sascha

Citation: Spudy, Bjorn; Soennichsen, Frank; Waetzig, Georg; Groetzinger, Joachim; Jung, Sascha. "Identification of structural traits that increase the antimicrobial activity of a chimeric peptide of human beta-defensins 2 and 3"  Biochem. Biophys. Res. Commun. 427, 207-211 (2012).

Assembly members:
entity, polymer, 44 residues, 4982.087 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: GIINTLQKYYCRVRGAICHP VFCPRRYKQIGTCGLPGTKC CKKP

Data sets:
Data typeCount
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Chimeric Peptide of HBD2 and HBD31

Entities:

Entity 1, Chimeric Peptide of HBD2 and HBD3 44 residues - 4982.087 Da.

1   GLYILEILEASNTHRLEUGLNLYSTYRTYR
2   CYSARGVALARGGLYALAILECYSHISPRO
3   VALPHECYSPROARGARGTYRLYSGLNILE
4   GLYTHRCYSGLYLEUPROGLYTHRLYSCYS
5   CYSLYSLYSPRO

Samples:

sample_1: HBD2_HBD3 0.15 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.02 M; pH: 4.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

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