BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18682

Title: LIP5-CHMP5   PubMed: 23105106

Authors: Skalicky, Jack; Sundquist, Wes

Citation: Skalicky, Jack; Arii, Jun; Wenzel, Dawn; Stubblefield, William-May; Katsuyama, Angela; Uter, Nathan; Bajorek, Monika; Myszka, David; Sundquist, Wesley. "Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport."  J. Biol. Chem. 287, 43910-43926 (2012).

Assembly members:
LIP5(1-168), polymer, 168 residues, 18833.916 Da.
CHMP5(139-195), polymer, 59 residues, 3797.847 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
LIP5(1-168): MAALAPLPPLPAQFKSIQHH LRTAQEHDKRDPVVAYYCRL YAMQTGMKIDSKTPECRKFL SKLMDQLEALKKQLGDNEAI TQEIVGCAHLENYALKMFLY ADNEDRAGRFHKNMIKSFYT ASLLIDVITVFGELTDENVK HRKYARWKATYIHNCLKNGE TPQAGPVG
CHMP5(139-195): GHMEDANEIQEALSRSYGTP ELDEDDLEAELDALGDELLA DEDSSYLDEAASAPAIPEG

Data sets:
Data typeCount
13C chemical shifts651
15N chemical shifts228
1H chemical shifts1397

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LIP5(1-168)1
2CHMP5(139-195)2

Entities:

Entity 1, LIP5(1-168) 168 residues - 18833.916 Da.

1   METALAALALEUALAPROLEUPROPROLEU
2   PROALAGLNPHELYSSERILEGLNHISHIS
3   LEUARGTHRALAGLNGLUHISASPLYSARG
4   ASPPROVALVALALATYRTYRCYSARGLEU
5   TYRALAMETGLNTHRGLYMETLYSILEASP
6   SERLYSTHRPROGLUCYSARGLYSPHELEU
7   SERLYSLEUMETASPGLNLEUGLUALALEU
8   LYSLYSGLNLEUGLYASPASNGLUALAILE
9   THRGLNGLUILEVALGLYCYSALAHISLEU
10   GLUASNTYRALALEULYSMETPHELEUTYR
11   ALAASPASNGLUASPARGALAGLYARGPHE
12   HISLYSASNMETILELYSSERPHETYRTHR
13   ALASERLEULEUILEASPVALILETHRVAL
14   PHEGLYGLULEUTHRASPGLUASNVALLYS
15   HISARGLYSTYRALAARGTRPLYSALATHR
16   TYRILEHISASNCYSLEULYSASNGLYGLU
17   THRPROGLNALAGLYPROVALGLY

Entity 2, CHMP5(139-195) 59 residues - 3797.847 Da.

1   GLYHISMETGLUASPALAASNGLUILEGLN
2   GLUALALEUSERARGSERTYRGLYTHRPRO
3   GLULEUASPGLUASPASPLEUGLUALAGLU
4   LEUASPALALEUGLYASPGLULEULEUALA
5   ASPGLUASPSERSERTYRLEUASPGLUALA
6   ALASERALAPROALAILEPROGLUGLY

Samples:

sample_1: LIP5(1-168), [U-100% 13C; U-100% 15N], 0.5 mM; CHMP5(139-195), [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 25 mM; sodium chloride 50 mM; DTT 0.5 mM; EDTA 0.1 mM; H2O 92%; D2O 8%

sample_conditions_1: ionic strength: 75 mM; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian DirectDrive 900 MHz

Related Database Links:

BMRB 18681
PDB
DBJ BAA90909 BAB22228 BAB26150 BAE43171 BAG73861 BAB25962 BAE27642 BAG37842 BAI46888
EMBL CAB66619 CAG33488 CAH92851 CAH90797
GB AAF76210 AAG43125 AAH05937 AAH06989 AAH22536 AAD27743 AAF29140 AAF42917 AAG23821 AAH06947
REF NP_001033134 NP_001126666 NP_001248449 NP_001273300 NP_057569 NP_001020581 NP_001029854 NP_001125453 NP_001231673 NP_001253497
SP Q32L63 Q5R5W5 Q9CR26 Q9NP79 Q4QQV8 Q5RBR3 Q9D7S9 Q9NZZ3
TPG DAA26071 DAA26641