BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18679

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for cold shock protein, LmCsp with dT7   PubMed: 23506337

Authors: Lee, Juho; Jeong, Ki-Woong; Kim, Yangmee

Citation: Lee, Juho; Jeong, Ki-Woong; Jin, Bonghwan; Ryu, Kyoung-Seok; Kim, Eun-Hee; Ahn, Joong-Hoon; Kim, Yangmee. "Structural and Dynamic Features of Cold-Shock Proteins of Listeriamonocytogenes, a Psychrophilic Bacterium"  Biochemistry 52, 2492-2504 (2013).

Assembly members:
LmCsp_dT7, polymer, 66 residues, 7272.949 Da.

Natural source:   Common Name: Firmicutes   Taxonomy ID: 1639   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Listeria monocytogenes

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
LmCsp_dT7: MEQGTVKWFNAEKGFGFIER ENGDDVFVHFSAIQGDGFKS LDEGQAVTFDVEEGQRGPQA ANVQKA

Data sets:
Data typeCount
13C chemical shifts232
15N chemical shifts73
1H chemical shifts387

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LmCsp_dT71

Entities:

Entity 1, LmCsp_dT7 66 residues - 7272.949 Da.

1   METGLUGLNGLYTHRVALLYSTRPPHEASN
2   ALAGLULYSGLYPHEGLYPHEILEGLUARG
3   GLUASNGLYASPASPVALPHEVALHISPHE
4   SERALAILEGLNGLYASPGLYPHELYSSER
5   LEUASPGLUGLYGLNALAVALTHRPHEASP
6   VALGLUGLUGLYGLNARGGLYPROGLNALA
7   ALAASNVALGLNLYSALA

Samples:

sample_1: LmCsp_dT7, [U-13C; U-15N], 0.8 mM; potassium phosphate 50 mM; potassium chloride 100 mM; EDTA 0.1 mM; DSS 0.004 v/v; H2O 90%; D2O 10%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CYANA, Brunger, Adams, Clore, Gros, Nilges and Read, Guntert, Mumenthaler and Wuthrich - chemical shift calculation, refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 18680
PDB
EMBL CAA62903 CAC20630 CAC96632 CAC99442 CAK20797
GB AAC80246 AAT04156 ACK39554 ADB68252 ADB71297
REF NP_464889 WP_003719639 WP_003756020 WP_012985572
SP P0A355 P0A356