BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18661

Title: STRUCTURE OF LASSO PEPTIDE CAULOSEGNIN I   PubMed: 23214991

Authors: Hegemann, J.; Zimmermann, M.; Xie, X.; Marahiel, M.

Citation: Hegemann, Julian; Zimmermann, Marcel; Xie, Xiulan; Marahiel, Mohamed. "Caulosegnins I-III: A Highly Diverse Group of Lasso Peptides Derived from a Single Biosynthetic Gene Cluster."  J. Am. Chem. Soc. 135, 210-222 (2013).

Assembly members:
CAULOSEGNIN_I, polymer, 19 residues, 1918.98 Da.

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 88688   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Caulobacter Segnis

Experimental source:   Production method: recombinant technology   Host organism: CAULOBACTER SEGNIS

Entity Sequences (FASTA):
CAULOSEGNIN_I: GAFVGQPEAVNPLGREIQG

Data sets:
Data typeCount
1H chemical shifts123

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CAULOSEGNIN I1

Entities:

Entity 1, CAULOSEGNIN I 19 residues - 1918.98 Da.

1   GLYALAPHEVALGLYGLNPROGLUALAVAL
2   ASNPROLEUGLYARGGLUILEGLNGLY

Samples:

sample_1: CAULOSEGNIN I 10 mM; methanol 100%

sample_conditions_1: ionic strength: . M; pH: .; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB ADG11022