BMRB Entry 18395

Title:
Chemical shift assignments of deuterated DsbB by 1H-detected solid-state NMR
Deposition date:
2012-04-13
Original release date:
2012-09-24
Authors:
Zhou, Donghua; Nieuwkoop, Andrew; Berthold, Deborah; Comellas, Gemma; Sperling, Lindsay; Tang, Ming; Shah, Gautam; Brea, Elliot; Lemkau, Luisel; Rienstra, Chad
Citation:

Citation: Zhou, Donghua; Nieuwkoop, Andrew; Berthold, Deborah; Comellas, Gemma; Sperling, Lindsay; Tang, Ming; Shah, Gautam; Brea, Elliott; Lemkau, Luisel; Rienstra, Chad. "Solid-state NMR analysis of membrane proteins and protein aggregates by proton detected spectroscopy."  J. Biomol. NMR 54, 291-305 (2012).
PubMed: 22986689

Assembly members:

Assembly members:
DsbB, polymer, 176 residues, 15287.664 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE70

Data sets:
Data typeCount
13C chemical shifts132
15N chemical shifts67
1H chemical shifts67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DsbB1

Entities:

Entity 1, DsbB 176 residues - 15287.664 Da.

1   METLEUARGPHELEUASNGLNALASERGLN
2   GLYARGGLYALATRPLEULEUMETALAPHE
3   THRALALEUALALEUGLULEUTHRALALEU
4   TRPPHEGLNHISVALMETLEULEULYSPRO
5   CYSVALLEUCYSILETYRGLUARGVALALA
6   LEUPHEGLYVALLEUGLYALAALALEUILE
7   GLYALAILEALAPROLYSTHRPROLEUARG
8   TYRVALALAMETVALILETRPLEUTYRSER
9   ALAPHEARGGLYVALGLNLEUTHRTYRGLU
10   HISTHRMETLEUGLNLEUTYRPROSERPRO
11   PHEALATHRCYSASPPHEMETVALARGPHE
12   PROGLUTRPLEUPROLEUASPLYSTRPVAL
13   PROGLNVALPHEVALALASERGLYASPCYS
14   ALAGLUARGGLNTRPASPPHELEUGLYLEU
15   GLUMETPROGLNTRPLEULEUGLYILEPHE
16   ILEALATYRLEUILEVALALAVALLEUVAL
17   VALILESERGLNPROPHELYSALALYSLYS
18   ARGASPLEUPHEGLYARG

Samples:

sample_1: DsbB, [U-100% 13C; U-100% 15N; U-80% 2H], 3 mg

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 248 K

Experiments:

NameSampleSample stateSample conditions
3D CANHsample_1isotropicsample_conditions_1
3D CONHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

BMRB 15546 15966 18493
PDB
DBJ BAA07408 BAA36032 BAB35103 BAG76757 BAI24997
EMBL CAP75720 CAQ31687 CAQ98064 CAR02574 CAR07527
GB AAA23711 AAB25233 AAC74269 AAG56036 AAN42789
PIR H85696
REF NP_309707 NP_415703 NP_707082 WP_000652474 WP_000943441
SP A1AAA8 P0A6M2 P0A6M3 P59343 Q0T5L6
AlphaFold P59343 P0A6M3 P0A6M2 A1AAA8 Q0T5L6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks