BMRB Entry 18368

Title:
Backbone 1H and 15N Chemical Shift Assignments for Hen Egg White Lysozyme mutant W108G.
Deposition date:
2012-03-29
Original release date:
2012-04-18
Authors:
Sziegat, Friederike; Silvers, Robert; Haehnke, Martin; Jensen, Malene; Blackledge, Martin; Wirmer-Bartoschek, Julia; Schwalbe, Harald
Citation:

Citation: Sziegat, Friederike; Silvers, Robert; Hahnke, Martin; Jensen, Malene Ringkjbing; Blackledge, Martin; Wirmer-Bartoschek, Julia; Schwalbe, Harald. "Disentangling the coil: modulation of conformational and dynamic properties by site-directed mutation in the non-native state of hen egg white lysozyme."  Biochemistry 51, 3361-3372 (2012).
PubMed: 22468860

Assembly members:

Assembly members:
W108G, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
15N chemical shifts121
1H chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1W108G1

Entities:

Entity 1, W108G 129 residues - Formula weight is not available

1   LYSVALPHEGLYARGALAGLULEUALAALA
2   ALAMETLYSARGHISGLYLEUASPASNTYR
3   ARGGLYTYRSERLEUGLYASNTRPVALALA
4   ALAALALYSPHEGLUSERASNPHEASNTHR
5   GLNALATHRASNARGASNTHRASPGLYSER
6   THRASPTYRGLYILELEUGLNILEASNSER
7   ARGTRPTRPALAASNASPGLYARGTHRPRO
8   GLYSERARGASNLEUALAASNILEPROALA
9   SERALALEULEUSERSERASPILETHRALA
10   SERVALASNALAALALYSLYSILEVALSER
11   ASPGLYASNGLYMETASNALAGLYVALALA
12   TRPARGASNARGALALYSGLYTHRASPVAL
13   GLNALATRPILEARGGLYALAARGLEU

Samples:

sample_1: W108G, [U-99% 15N], 300 uM; H2O 49.95 M; D2O, [U-2H], 5.55 M

sample_conditions_1: pH: 2.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 11051 11459 11460 11461 11462 15198 18365 18366 18367 18369 18370

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks