BMRB Entry 18207

Title:
A53T alpha-synuclein fibrils
Deposition date:
2012-01-22
Original release date:
2013-04-02
Authors:
Lemkau, Luisel; Comellas, Gemma; Lee, Shin; Rikardsen, Lars; Woods, Wendy; George, Julia; Rienstra, Chad
Citation:

Citation: Lemkau, Luisel; Comellas, Gemma; Lee, Shin; Rikardsen, Lars; Woods, Wendy; George, Julia; Rienstra, Chad. "Site-Specific Perturbations of Alpha-Synuclein Fibril Structure by the Parkinson's Disease Associated Mutations A53T and E46K."  PLoS ONE 8, e49750-e49750 (2013).
PubMed: 23505409

Assembly members:

Assembly members:
A53T_alpha-synuclein_fibrils, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: DE3 peT28

Data sets:
Data typeCount
13C chemical shifts185
15N chemical shifts52

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1A53T AS1

Entities:

Entity 1, A53T AS 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLYSSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALTHRTHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: A53T alpha-synuclein fibrils, [U-13C; U-15N], 36%; H2O 100%

sample_conditions_1: pH: 7.4; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
CAN(co)CXsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16904 16939 17214 17498 17648 17649 17654 17665 17910 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228
PDB
DBJ BAB29375 BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001032222 NP_001129014 NP_001139526
SP P37840 P61139 P61140 P61142 P61143
AlphaFold P61143 P37840 P61139 P61140 P61142