BMRB Entry 18116

Title:
backbone and sidechain order parameters for ubiquitin at 1, 400, 800, 1200, 1600 and 2500 bar
Deposition date:
2011-12-01
Original release date:
2019-08-29
Authors:
Fu, Yinan; Kasinath, Vignesh; Moorman, Veronica; Nucci, Nathaniel; Hilser, Vincent; Wand, A
Citation:

Citation: Fu, Yinan; Kasinath, Vignesh; Moorman, Veronica; Nucci, Nathaniel; Hilser, Vincent; Wand, A Joshua. "Coupled motion in proteins revealed by pressure perturbation"  J. Am. Chem. Soc. 134, 8543-8550 (2012).
PubMed: 22452540

Assembly members:

Assembly members:
ubiquitin, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Data typeCount
order parameters546

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin1

Entities:

Entity 1, ubiquitin 76 residues - Formula weight is not available

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: ubiquitin, [U-100% 13C; U-55% 2H], 1.2 mM; ubiquitin, [U-100% 15N], 0.8 mM; sodium chloride 50 mM; sodium acetate 50 mM; sodium azide 3 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 5.0; pressure: 1 bar; temperature: 273 K

sample_conditions_2: ionic strength: 100 mM; pH: 5.0; pressure: 400 bar; temperature: 273 K

sample_conditions_3: ionic strength: 100 mM; pH: 5.0; pressure: 800 bar; temperature: 273 K

sample_conditions_4: ionic strength: 100 mM; pH: 5.0; pressure: 1200 bar; temperature: 273 K

sample_conditions_5: ionic strength: 100 mM; pH: 5.0; pressure: 1600 bar; temperature: 273 K

sample_conditions_6: ionic strength: 100 mM; pH: 5.0; pressure: 2500 bar; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
15N relaxationsample_1isotropicsample_conditions_1
2H relaxationsample_1isotropicsample_conditions_1
15N relaxationsample_1isotropicsample_conditions_2
2H relaxationsample_1isotropicsample_conditions_2
15N relaxationsample_1isotropicsample_conditions_3
2H relaxationsample_1isotropicsample_conditions_3
15N relaxationsample_1isotropicsample_conditions_4
2H relaxationsample_1isotropicsample_conditions_4
15N relaxationsample_1isotropicsample_conditions_5
2H relaxationsample_1isotropicsample_conditions_5
15N relaxationsample_1isotropicsample_conditions_6
2H relaxationsample_1isotropicsample_conditions_6

Software:

FELIX, Accelrys Software Inc. - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 750 MHz