BMRB Entry 18004

Title:
Structural and functional analysis of the DEAF-1 and BS69 MYND domains
Deposition date:
2011-10-18
Original release date:
2012-11-05
Authors:
Kateb, F.; Perrin, H.; Tripsianes, Kostantinos; Zou, P.; Spadaccini, R.; Bottomley, M.; Bepperling, A.; Ansieau, S.; Sattler, M.
Citation:

Citation: Kateb, F.; Perrin, H.; Tripsianes, Kostantinos; Zou, P.; Spadaccini, R.; Bottomley, M.; Bepperling, A.; Ansieau, S.; Sattler, M.. "Structural and functional analysis of the DEAF-1 and BS69 MYND domains"  J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
DEFORMED_EPIDERMAL_AUTOREGULATORY_FACTOR_1_HOMOLO, polymer, 47 residues, 5262.8434 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETMThx-MYND

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DEFORMED_EPIDERMAL_AUTOREGULATORY_FACTOR_1_HOMOLO: GAMEQSCVNCGREAMSECTG CHKVNYCSTFCQRKDWKDHQ HICGQSA

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts52
1H chemical shifts280

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DEFORMED EPIDERMAL AUTOREGULATORY FACTOR 1 HOMOLO1
2ZINC ION2
3ZINC ION2

Entities:

Entity 1, DEFORMED EPIDERMAL AUTOREGULATORY FACTOR 1 HOMOLO 47 residues - 5262.8434 Da.

1   GLYALAMETGLUGLNSERCYSVALASNCYS
2   GLYARGGLUALAMETSERGLUCYSTHRGLY
3   CYSHISLYSVALASNTYRCYSSERTHRPHE
4   CYSGLNARGLYSASPTRPLYSASPHISGLN
5   HISILECYSGLYGLNSERALA

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: DEFORMED EPIDERMAL AUTOREGULATORY FACTOR 1 HOMOLO, [U-13C; U-15N], 1 mM; H2O 10%; D2O 90%; PO4 50 mM; NaCl 100 mM; DTT 2 mM

sample_conditions_1: ionic strength: 100.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 295.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1solutionsample_conditions_1
3D 1H-15N NOESYsample_1solutionsample_conditions_1
3D 1H-13C NOESY (aliphatic carbons)sample_1solutionsample_conditions_1
3D 1H-13C NOESY (aromatic carbons)sample_1solutionsample_conditions_1
3D 1H-15N NOESYsample_1solutionsample_conditions_1
3D 1H-13C NOESY (ALIPHATIC CARBONS)sample_1solutionsample_conditions_1
3D 1H-13C NOESY (AROMATIC CARBONS)sample_1solutionsample_conditions_1

Software:

ARIA-CNS vany, Linge, O'Donoghue and Nilges - chemical shift assignment

AutoDep v4.3, AutoDep - chemical shift assignment

CNS-ARIA vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

SPARKY vany, Goddard - collection

TALOS vany, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

UNP DEAF1_HUMAN
PDB
DBJ BAD93068 BAE27251 BAF82562 BAF84072 BAH24132
GB AAB62704 AAC25716 AAC25719 AAC79676 AAC79677
REF NP_001008974 NP_001269001 NP_058570 NP_066288 NP_113989
SP O75398 O77562 O88450 Q9Z1T5
AlphaFold Q9UET1 O88450 O75398 O77562 Q9Z1T5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks