BMRB Entry 17992

Title:
WSA minor conformation
Deposition date:
2011-10-11
Original release date:
2012-01-09
Authors:
Xu, Yan; Mowrey, David; Cui, Tanxing; Perez-Aguilar, Jose; Saven, Jeffrey; Eckenhoff, Roderic; Tang, Pei
Citation:

Citation: Cui, Tanxing; Mowrey, David; Bondarenko, Vasyl; Tillman, Tommy; Ma, Dejian; Landrum, Elizabeth; Perez-Aguilar, Jose Manuel; He, Jing; Wang, Wei; Saven, Jeffery; Eckenhoff, Roderic; Tang, Pei; Xu, Yan. "NMR structure and dynamics of a designed water-soluble transmembrane domain of nicotinic acetylcholine receptor."  Biochim. Biophys. Acta 1818, 617-626 (2012).
PubMed: 22155685

Assembly members:

Assembly members:
Nicotinic_Acetylcholine_Receptor, polymer, 140 residues, 15558.247 Da.

Natural source:

Natural source:   Common Name: Marbled electric ray   Taxonomy ID: 7788   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Torpedo marmorata

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: NA

Data sets:
Data typeCount
13C chemical shifts334
15N chemical shifts127
1H chemical shifts845

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nicotinic_Acetylcholine_Receptor1

Entities:

Entity 1, Nicotinic_Acetylcholine_Receptor 140 residues - 15558.247 Da.

1   METALAHISHISHISHISHISHISALAMET
2   VALILEASPHISILELEULYSCYSVALPHE
3   ASPLYSILECYSLYSILEGLYTHRGLUSER
4   VALGLUALAGLYARGLEUILEGLULEUSER
5   GLNGLUGLYGLYGLYGLYGLYGLYPROLEU
6   TYRPHEVALVALASNVALILEGLUPROCYS
7   LYSLYSPHESERGLULEUTHRGLYLEUVAL
8   PHETYRLEUPROTHRASPSERGLYGLULYS
9   METTHRGLUSERLYSSERVALLEULYSSER
10   LEUTHRGLULYSLEULYSLYSILEVALGLU
11   LEUILEPROSERTHRSERSERALAVALPRO
12   LEUILEGLYLYSTYRMETLEUPHETHRLYS
13   GLUPHEVALGLUSERSERILELYSILETHR
14   GLUGLUVALILEASNTHRHISHISARGSER

Samples:

sample_1: sodium phosphate 20 mM; LPPG 2%; D2O, [U-100% 2H], 10%; H2O, [U-100% 2H], 90%; Nicotinic_Acetylcholine_Receptor, [U-100% 2H], 0.6 mM; 2-Mercaptoethanol, [U-100% 2H], 10 mM

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 17991
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks