BMRB Entry 17913

Title:
The third SH3 domain of R85FL with ataxin-7 PRR
Deposition date:
2011-09-02
Original release date:
2012-09-04
Authors:
Jiang, Ya-Jun; Hu, Hong-Yu
Citation:

Citation: Jiang, Ya-Jun; Zhou, Chen-Jie; Zhou, Zi-Ren; Wu, Meng; Hu, Hong-Yu. "Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7."  FEBS Lett. 587, 2905-2911 (2013).
PubMed: 23892081

Assembly members:

Assembly members:
SH3C, polymer, 64 residues, 7468.242 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts186
15N chemical shifts61
1H chemical shifts311

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3C1

Entities:

Entity 1, SH3C 64 residues - 7468.242 Da.

1   GLNTHRSERGLNASPLEUPHESERTYRGLN
2   ALALEUTYRSERTYRILEPROGLNASNASP
3   ASPGLULEUGLULEUARGASPGLYASPILE
4   VALASPVALMETGLULYSCYSASPASPGLY
5   TRPPHEVALGLYTHRSERARGARGTHRLYS
6   GLNPHEGLYTHRPHEPROGLYASNTYRVAL
7   LYSPROLEUTYR

Samples:

sample_1: sodium phosphate 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.12 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17912
PDB
DBJ BAA92534 BAG58006 BAG62137 BAG65026 BAG72789
EMBL CAB55947 CAD34588 CAE45892 CAH89601 CAH90184
GB AAD27647 AAF22175 AAI52464 AAI60134 AAK37563
REF NP_001030126 NP_001030127 NP_001030128 NP_001030129 NP_001068637
SP Q9BX66
AlphaFold Q9BX66

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks