BMRB Entry 17904

Title:
NMR Solution Structure of Yeast Iso-1-cytochrome c Mutant P71H in reduced states
Deposition date:
2011-08-31
Original release date:
2011-12-06
Authors:
Lan, Wenxian; Wang, Zhonghua; Yang, Zhongzheng; Zhu, Jing; Ying, Tianlei; Jiang, Xianwang; Zhang, Xu; Wu, Houming; Liu, Maili; Tan, Xiangshi; Cao, Chunyang; Huang, Zhong-xian
Citation:

Citation: Lan, Wenxian; Wang, Zhonghua; Yang, Zhongzheng; Zhu, Jing; Ying, Tianlei; Jiang, Xianwang; Zhang, Xu; Wu, Houming; Liu, Maili; Tan, Xiangshi; Cao, Chunyang; Huang, Zhong-xian. "Conformational toggling of Yeast Iso-1-cytochrome c in the oxidized and reduced states"  PLoS One 6, e27219-. (2011).
PubMed: 22087268

Assembly members:

Assembly members:
Iso-1-cytochrome, polymer, 108 residues, 12054.826 Da.
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBTR2

Data sets:
Data typeCount
1H chemical shifts616

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Iso-1-cytochrome1
2HEC2

Entities:

Entity 1, Iso-1-cytochrome 108 residues - 12054.826 Da.

1   THRGLUPHELYSALAGLYSERALALYSLYS
2   GLYALATHRLEUPHELYSTHRARGCYSLEU
3   GLNCYSHISTHRVALGLULYSGLYGLYPRO
4   HISLYSVALGLYPROASNLEUHISGLYILE
5   PHEGLYARGHISSERGLYGLNALAGLUGLY
6   TYRSERTYRTHRASPALAASNILELYSLYS
7   ASNVALLEUTRPASPGLUASNASNMETSER
8   GLUTYRLEUTHRASNHISALALYSTYRILE
9   PROGLYTHRLYSMETALAPHEGLYGLYLEU
10   LYSLYSGLULYSASPARGASNASPLEUILE
11   THRTYRLEULYSLYSALATHRGLU

Entity 2, HEC - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: Iso-1-cytochrome 1 mM; H2O 90%; D2O 10%

sample_2: Iso-1-cytochrome 1 mM; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D DQF-COSYsample_2anisotropicsample_conditions_1
2D 1H-1H NOESYsample_2anisotropicsample_conditions_1
2D 1H-1H TOCSYsample_2anisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 131 15301 17845 17846 17903 19638
PDB
DBJ GAA24396
EMBL CAA24605 CAA89576 CAY80764
GB AAA62856 AAA88751 AAB59344 AHY79034 AJP39735
PRF 1409323A
REF NP_012582
SP P00044
TPG DAA08835
AlphaFold P00044