BMRB Entry 17715

Title:
Itch WW domain 2
Deposition date:
2011-06-16
Original release date:
2019-08-29
Authors:
Barbato, Gaetano; Bellomaria, Alessia; Melino, Sonia
Citation:

Citation: Bellomaria, Alessia; Melino, Sonia; Barbato, Gaetano; Paci, Maurizio; Melino, Gerry. "Itch-E3 ligase second WW domain 1H-13C-15N assignments"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
Itch_WW2, polymer, 49 residues, 5000 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: none

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Itch_WW2: GPLGSDRPEPLPPGWERRVD NMGRIYYVDHFTRTTTWQRP TLESVRNYE

Data sets:
Data typeCount
13C chemical shifts187
15N chemical shifts44
1H chemical shifts267

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WW21

Entities:

Entity 1, WW2 49 residues - 5000 Da.

1   GLYPROLEUGLYSERASPARGPROGLUPRO
2   LEUPROPROGLYTRPGLUARGARGVALASP
3   ASNMETGLYARGILETYRTYRVALASPHIS
4   PHETHRARGTHRTHRTHRTRPGLNARGPRO
5   THRLEUGLUSERVALARGASNTYRGLU

Samples:

sample_1: Itch WW2, [U-100% 13C; U-100% 15N], 0.6 mM; potassium chloride 50 mM; NaPi 10 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRView vNMRViewJ, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks