BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17395

Title: The 27-residue N- terminus CCR5-peptide in a ternary complex with HIV-1 gp120 and a CD4-mimic peptide   PubMed: 21763489

Authors: Schnur, Einat; Noah, Eran; Ayzenshtat, Inbal; Sargsyan, Hasmik; Inuib, Tatsuya; Ding, Fa-Xiang; Arshava, Boris; Sagi, Yael; Kessler, Naama; Levy, Rina; Scherf, Tali; Naider, Fred; Anglister, Jacob

Citation: Schnur, Einat; Noah, Eran; Ayzenshtat, Inbal; Sargsyan, Hasmik; Inui, Tatsuya; Ding, Fa-Xiang; Arshava, Boris; Sagi, Yael; Kessler, Naama; Levy, Rina; Scherf, Tali; Naider, Fred; Anglister, Jacob. "The conformation and orientation of a 27-residue CCR5 peptide in a ternary complex with HIV-1 gp120 and a CD4-mimic peptide."  J. Mol. Biol. 410, 778-797 (2011).

Assembly members:
CCR5, polymer, 27 residues, 3357.671 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
CCR5: MDYQVSSPIXDINXYTSEPA QKINVKQ

Data sets:
Data typeCount
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CCR51

Entities:

Entity 1, CCR5 27 residues - 3357.671 Da.

1   METASPTYRGLNVALSERSERPROILETYS
2   ASPILEASNTYSTYRTHRSERGLUPROALA
3   GLNLYSILEASNVALLYSGLN

Samples:

sample_1: Nt-CCR5 1 mM; gp120/CD4mimic 0.1 mM; H2O 95%; H2O 5%

pH_7.0_300_mM_NaCl: ionic strength: 300 mM; pH: 7.0

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicpH_7.0_300_mM_NaCl

Software:

NMRView, Johnson, One Moon Scientific - peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

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