BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17394

Title: Solution NMR structure of human amylin in SDS micelles at pH 7.3   PubMed: 21723249

Authors: Nanga, Ravi Prakash Reddy; Brender, Jeffrey; Vivekanandan, Subramanian; Ramamoorthy, Ayyalusamy

Citation: Nanga, Ravi Prakash Reddy; Brender, Jeffrey; Vivekanandan, Subramanian; Ramamoorthy, Ayyalusamy. "Structure and membrane orientation of IAPP in its natively amidated form at physiological pH in a membrane environment."  Biochim. Biophys. Acta 1808, 2337-2342 (2011).

Assembly members:
entity, polymer, 37 residues, 3909.330 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):
entity: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY

Data sets:
Data typeCount
1H chemical shifts228

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1amidated IAPP1

Entities:

Entity 1, amidated IAPP 37 residues - 3909.330 Da.

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALHISSERSER
3   ASNASNPHEGLYALAILELEUSERSERTHR
4   ASNVALGLYSERASNTHRTYR

Samples:

sample_1: amidated IAPP 2.5 mM; sodium phosphate 20 mM; sodium chloride 120 mM; D2O, [U-99% 2H], 10%; H2O 90%; SDS, [U-99% 2H], 200 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis, structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

BMRB 16104 16105 18795 20045
PDB
DBJ BAG73319
EMBL CAA33032 CAA37002 CAA39504 CAA48724 CAB57803
GB AAA35524 AAA35983 AAA51728 AAA52281 AAI11850
REF NP_000406 XP_001144800 XP_003265632 XP_003265633 XP_003828947
SP P10997