BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17192

Title: Solution NMR Structure of CH domain of Rho guanine nucleotide exchange factor 7 from Homo sapiens, Northeast Structural Genomics Consortium Target HR4495E

Authors: Liu, Gaohua; Xiao, Rong; Janjua, Haleema; Acton, Thomas; Ciccosanti, Colleen; Shastry, Ritu; Everett, John; Montelione, Gaetano

Citation: Liu, Gaohua; Xiao, Rong; Janjua, Haleema; Ciccosanti, Colleen; Shastry, Ritu; Acton, Thomas; Everett, John; Montelione, Gaetano. "Northeast Structural Genomics Consortium Target HR4495E"  To be published ., .-..

Assembly members:
HR4495E, polymer, 126 residues, 14070.131 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HR4495E: MGHHHHHHSHMNSAEQTVTW LITLGVLESPKKTISDPEGF LQASLKDGVVLCRLLERLLP GTIEKVYPEPRSESECLSNI REFLRGCGASLRLETFDAND LYQGQNFNKVLSSLVTLNKV TADIGL

Data sets:
Data typeCount
13C chemical shifts518
15N chemical shifts128
1H chemical shifts856

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HR4495E1

Entities:

Entity 1, HR4495E 126 residues - 14070.131 Da.

sequence contains N-terminal His-tag: MGHHHHHHSHM

1   METGLYHISHISHISHISHISHISSERHIS
2   METASNSERALAGLUGLNTHRVALTHRTRP
3   LEUILETHRLEUGLYVALLEUGLUSERPRO
4   LYSLYSTHRILESERASPPROGLUGLYPHE
5   LEUGLNALASERLEULYSASPGLYVALVAL
6   LEUCYSARGLEULEUGLUARGLEULEUPRO
7   GLYTHRILEGLULYSVALTYRPROGLUPRO
8   ARGSERGLUSERGLUCYSLEUSERASNILE
9   ARGGLUPHELEUARGGLYCYSGLYALASER
10   LEUARGLEUGLUTHRPHEASPALAASNASP
11   LEUTYRGLNGLYGLNASNPHEASNLYSVAL
12   LEUSERSERLEUVALTHRLEUASNLYSVAL
13   THRALAASPILEGLYLEU

Samples:

sample_NC: HR4495E, [U-100% 13C; U-100% 15N], 0.87 mM; H2O 95%; D2O 5%

sample_NC5: HR4495E, [U-5% 13C; U-100% 15N], 0.85 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_NCisotropicsample_conditions_1
2D 1H-13C HSQC ct 28sample_NC5isotropicsample_conditions_1
3D HNCOsample_NCisotropicsample_conditions_1
3D CBCA(CO)NHsample_NCisotropicsample_conditions_1
3D HNCACBsample_NCisotropicsample_conditions_1
3D 1H-13C arom NOESYsample_NCisotropicsample_conditions_1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESYsample_NCisotropicsample_conditions_1
2D 1H-13C HSQC ct 42sample_NC5isotropicsample_conditions_1
3D HBHA(CO)NHsample_NCisotropicsample_conditions_1
3D CC(CO)NH TOCSYsample_NCisotropicsample_conditions_1
2D 1H-15N HSQCsample_NCisotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, refinemen, structure solution

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinemen, structure solution

AutoStruct v2.1, Huang, Tejero, Powers and Montelione - data analysis, refinement

AutoAssign v2.1, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

TALOS+, Shen, Cornilescu, Delaglio and Bax - geometry optimization

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAC97874 BAE23905
EMBL CAD38906
GB ACE87830 ACT64348 EAX09145 EAX09148 ELK14885
REF NP_001106989 NP_663788 XP_001365011 XP_003778289 XP_004054793
SP Q9ES28