BMRB Entry 16767

Title:
Human Regenerating Gene Type IV (REG IV) PROTEIN, P91S mutant
Deposition date:
2010-03-04
Original release date:
2010-08-12
Authors:
Ho, Meng-Ru; Lou, Yuan-Chao; Chen, Chinpan
Citation:

Citation: Ho, Meng-Ru; Lou, Yuan-Chao; Wei, Shu-Yi; Luo, Shih-Chi; Lin, Wen-Chang; Lyu, Ping-Chiang; Chen, Chinpan. "Human RegIV Protein Adopts a Typical C-Type Lectin Fold but Binds Mannan with Two Calcium-Independent Sites."  J. Mol. Biol. 402, 682-695 (2010).
PubMed: 20692269

Assembly members:

Assembly members:
P91S, polymer, 130 residues, 16301 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE1

Data sets:
Data typeCount
13C chemical shifts523
15N chemical shifts145
1H chemical shifts805

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P91S1

Entities:

Entity 1, P91S 130 residues - 16301 Da.

1   GLNSERCYSALAPROGLYTRPPHETYRHIS
2   LYSSERASNCYSTYRGLYTYRPHEARGLYS
3   LEUARGASNTRPSERASPALAGLULEUGLU
4   CYSGLNSERTYRGLYASNGLYALAHISLEU
5   ALASERILELEUSERLEULYSGLUALASER
6   THRILEALAGLUTYRILESERGLYTYRGLN
7   ARGSERGLNSERILETRPILEGLYLEUHIS
8   ASPPROGLNLYSARGGLNGLNTRPGLNTRP
9   ILEASPGLYALAMETTYRLEUTYRARGSER
10   TRPSERGLYLYSSERMETGLYGLYASNLYS
11   HISCYSALAGLUMETSERSERASNASNASN
12   PHELEUTHRTRPSERSERASNGLUCYSASN
13   LYSARGGLNHISPHELEUCYSLYSTYRARG

Samples:

sample_1: Human Regenerating Gene Type IV, [U-99% 15N], 1 mM; Human Regenerating Gene Type IV, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 4.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAG02562 AAH17089 AAK48435 AAK59869 AAM95598
REF NP_001152824 NP_114433 XP_001139975 XP_003268113 XP_003268114
SP Q9BYZ8
AlphaFold Q9BYZ8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks