BMRB Entry 16278

Title:
NMR structure of the H103G mutant SO2144 H-NOX domain from Shewanella oneidensis in the Fe(II)CO ligation state
Deposition date:
2009-05-06
Original release date:
2010-05-06
Authors:
Erbil, William
Citation:

Citation: Erbil, W. Kaya; Price, Mark; Wemmer, David; Marletta, Michael. "A structural basis for H-NOX signaling in Shewanella oneidensis by trapping a histidine kinase inhibitory conformation."  Proc. Natl. Acad. Sci. U.S.A. 106, 19753-19760 (2009).
PubMed: 19918063

Assembly members:

Assembly members:
SO2144, polymer, 181 residues, 20453.637 Da.
CMO, non-polymer, 28.010 Da.
HEM, non-polymer, 616.487 Da.
IMD, non-polymer, 69.085 Da.

Natural source:

Natural source:   Common Name: Shewanella oneidensis MR-1   Taxonomy ID: 211586   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shewanella oneidensis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET20b

Data sets:
Data typeCount
13C chemical shifts502
15N chemical shifts167
1H chemical shifts1149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SO21441
2CMO2
3HEM3
4IMD4

Entities:

Entity 1, SO2144 181 residues - 20453.637 Da.

1   METLYSGLYILEILEPHEASNVALLEUGLU
2   ASPMETVALVALALAGLNCYSGLYMETSER
3   VALTRPASNGLULEULEUGLULYSHISALA
4   PROLYSASPARGVALTYRVALSERALALYS
5   SERTYRALAGLUSERGLULEUPHESERILE
6   VALGLNASPVALALAGLNARGLEUASNMET
7   PROILEGLNASPVALVALLYSALAPHEGLY
8   GLNPHELEUPHEASNGLYLEUALASERARG
9   HISTHRASPVALVALASPLYSPHEASPASP
10   PHETHRSERLEUVALMETGLYILEHISASP
11   VALILEGLYLEUGLUVALASNLYSLEUTYR
12   HISGLUPROSERLEUPROHISILEASNGLY
13   GLNLEULEUPROASNASNGLNILEALALEU
14   ARGTYRSERSERPROARGARGLEUCYSPHE
15   CYSALAGLUGLYLEULEUPHEGLYALAALA
16   GLNHISPHEGLNGLNLYSILEGLNILESER
17   HISASPTHRCYSMETHISTHRGLYALAASP
18   HISCYSMETLEUILEILEGLULEUGLNASN
19   ASP

Entity 2, CMO - C O - 28.010 Da.

1   CMO

Entity 3, HEM - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Entity 4, IMD - C3 H5 N2 - 69.085 Da.

1   IMD

Samples:

sample_1: H-NOX, [U-99% 13C; U-99% 15N], 0.4 – 0.8 mM; K3PO4 50 mM; DTT 5 mM; glycerol 5%; H2O 90%; D2O 10%; imidazole 10 mM

sample_2: H-NOX, [U-99% 13C; U-99% 15N], 0.4 – 0.8 mM; K3PO4 50 mM; DTT 5 mM; glycerol 5%; D2O 100%; imidazole 10 mM

sample_3: H-NOX, [U-99% 13C; U-99% 15N], 0.4 – 0.8 mM; K3PO4 50 mM; DTT 5 mM; glycerol 5%; H2O 90%; D2O 10%; imidazole 10 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D 1H-15N TOCSYsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker DRX 900 MHz
  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 16276
PDB
GB AAN55189
REF NP_717745 WP_011072197

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks