BMRB Entry 16082

Name: solution structure of FAPP1 PH domain
Published: 2010-01-07

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PDB ID: 2kcj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16082
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

solution structure of FAPP1 PH domain

Deposition date:

2008-12-22

Original release date:

2010-01-07

Authors:

Lenoir, Marc; Coskun, Uenal; James, Jonathan; Simons, Kai; Overduin, Michael

Citation:

Citation: He, Ju; Scott, Jordan; Heroux, Annie; Roy, Siddhartha; Lenoir, Marc; Overduin, Michael; Stahelin, Robert; Kutateladze, Tatiana. "Molecular basis of phosphatidylinositol 4-phosphate and ARF1 GTPase recognition by the FAPP1 pleckstrin homology (PH) domain." J. Biol. Chem. 286, 18650-18657 (2011).
PubMed: 21454700

Assembly members:

Assembly members:
FAPP1-PH, polymer, 108 residues, 12219.981 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FAPP1-PH: GPLGSPEFMEGVLYKWTNYL TGWQPRWFVLDNGILSYYDS QDDVCKGSKGSIKMAVCEIK VHSADNTRMELIIPGEQHFY MKAVNAAERQRWLVALGSSK ASLTDTRT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	465
15N chemical shifts	117
1H chemical shifts	746

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FAPP1-PH	1

Entities:

Entity 1, FAPP1-PH 108 residues - 12219.981 Da.

1

GLY

PRO

LEU

GLY

SER

PRO

GLU

PHE

MET

GLU

2

GLY

VAL

LEU

TYR

LYS

TRP

THR

ASN

TYR

LEU

3

THR

GLY

TRP

GLN

PRO

ARG

TRP

PHE

VAL

LEU

4

ASP

ASN

GLY

ILE

LEU

SER

TYR

ASP

SER

5

GLN

ASP

VAL

CYS

LYS

GLY

SER

LYS

GLY

6

SER

ILE

LYS

MET

ALA

VAL

CYS

GLU

ILE

LYS

7

VAL

HIS

SER

ALA

ASP

ASN

THR

ARG

MET

GLU

8

LEU

ILE

PRO

GLY

GLU

GLN

HIS

PHE

TYR

9

MET

LYS

ALA

VAL

ASN

ALA

GLU

ARG

GLN

10

ARG

TRP

LEU

VAL

ALA

LEU

GLY

SER

LYS

11

ALA

SER

LEU

THR

ASP

THR

ARG

THR

Samples:

sample_1: FAPP1-PH, [U-13C; U-15N], 600 uM; TRIS 20 mM; sodium azide 1 mM; sodium chloride 100 mM

sample_2: FAPP1-PH, [U-15N], 600 uM; TRIS 20 mM; sodium azide 1 mM; sodium chloride 100 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

ARIA v2, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

Varian INOVA 800 MHz

BMRB	19508 19576
PDB	2KCJ 2MDX 3RCP
DBJ	BAG37067
GB	AAG15199 AAG15200 AAH31110 AAH44567 AAH83759
REF	NP_001013095 NP_001095383 NP_001230393 NP_061964 NP_112546
SP	Q9ERS4 Q9HB20
TPG	DAA32821
AlphaFold	Q9HB20 Q9ERS4